(1)
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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0320, RRID:AB_2833483.
展开/折叠
E3 ubiquitin protein ligase TRIM24; E3 ubiquitin-protein ligase Trim24; hTIF1; PTC6; RING finger protein 82; RNF82; TF1A; TIF1; TIF1-alpha; TIF1A; TIF1A_HUMAN; TIF1ALPHA; Transcription intermediary factor 1-alpha; Transcriptional intermediary factor 1 alpha; Transcriptional intermediary factor 1; Trim24; Tripartite motif containing 24; Tripartite motif-containing protein 24;
抗原和靶标
- O15164 TIF1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERGGEAARLNLLDTCAVCHQNIQSRAPKLLPCLHSFCQRCLPAPQRYLMLPAPMLGSAETPPPVPAPGSPVSGSSPFATQVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKTKYIKFTGNQIQNRIIEVNQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNNTIQFHCDPSFWAQNIINLGSLVIEDKESQPQMPKQNPVVEQNSQPPSGLSSNQLSKFPTQISLAQLRLQHMQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQQPPPRLINFQNHSPKPNGPVLPPHPQQLRYPPNQNIPRQAIKPNPLQMAFLAQQAIKQWQISSGQGTPSTTNSTSSTPSSPTITSAAGYDGKAFGSPMIDLSSPVGGSYNLPSLPDIDCSSTIMLDNIVRKDTNIDHGQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQSSTSEETVLRSDAPDSTGDQPGLHQDNSSNGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYSKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESEDNKFSDDSDDDFVQPRKKRLKSIEERQLLK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15164 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S41 | Phosphorylation | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| R469 | Methylation | Uniprot | |
| R481 | Methylation | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| K641 | Ubiquitination | Uniprot | |
| S654 | Phosphorylation | Uniprot | |
| T657 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| S663 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| S667 | Phosphorylation | Uniprot | |
| S685 | Phosphorylation | Uniprot | |
| S687 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| K702 | Sumoylation | Uniprot | |
| K711 | Sumoylation | Uniprot | |
| K723 | Sumoylation | Uniprot | |
| Y733 | Phosphorylation | Uniprot | |
| K741 | Sumoylation | Uniprot | |
| S744 | Phosphorylation | Uniprot | |
| S748 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| S768 | Phosphorylation | Uniprot | |
| S771 | Phosphorylation | Uniprot | |
| T772 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | Uniprot | |
| T776 | Phosphorylation | Uniprot | |
| T786 | Phosphorylation | Uniprot | |
| S797 | Phosphorylation | Uniprot | |
| K801 | Sumoylation | Uniprot | |
| S808 | Phosphorylation | Uniprot | |
| S811 | Phosphorylation | Uniprot | |
| T818 | Phosphorylation | Uniprot | |
| S874 | Phosphorylation | Uniprot | |
| Y916 | Phosphorylation | Uniprot | |
| Y936 | Phosphorylation | Uniprot | |
| K940 | Sumoylation | Uniprot | |
| K949 | Sumoylation | Uniprot | |
| K992 | Sumoylation | Uniprot | |
| Y996 | Phosphorylation | Uniprot | |
| K1002 | Ubiquitination | Uniprot | |
| S1019 | Phosphorylation | Uniprot | |
| S1025 | Phosphorylation | Uniprot | |
| S1028 | Phosphorylation | Uniprot | |
| S1042 | Phosphorylation | Uniprot |
研究背景
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity).
Sumoylated.
Nucleus. Cytoplasm.
Note: Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.
Interacts with CARM1, NCOA2/GRIP1, PML, KAT5/TIP60, BRD7, CBX1, CBX3 and CBX5. Part of a coactivator complex containing TRIM24, NCOA2 and CARM1 (By similarity). Interacts with NR3C2/MCR. Interacts with the ligand-binding domain of estrogen receptors (in vitro). Interaction with DNA-bound estrogen receptors requires the presence of estradiol. Interacts with AR and p53/TP53. Interacts (via bromo domain) with histone H3 (via N-terminus), provided that it is not methylated at 'Lys-4' (H3K4me0). Does not interact with histone H3 that is methylated at 'Lys-4' (H3K4me1, H3K4me2 or H3K4me3). Interacts (via bromo domain) with histone H3 (via N-terminus) that is acetylated at 'Lys-23' (H3K23ac). Has the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has very low affinity for histone H3 that is methylated at 'Lys-9' (H3K9me), or acetylated at both 'Lys-9' (H3K9ac) and 'Lys-14' (H3K14ac), or acetylated at 'Lys-27' (H3K27ac) (in vitro). Interacts with TRIM16.
文献引用
Application: WB Species: Human Sample: ectopic endometrial tissues
Application: IHC Species: Human Sample: ectopic endometrial tissues
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