PKA R2 Antibody - #DF3091

(5)  
产品: PKA R2 抗体
货号: DF3091
描述: Rabbit polyclonal antibody to PKA R2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Dog
分子量: 45 KD; 46kD(Calculated).
蛋白号: P13861
RRID: AB_2835474

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Dog(89%)
克隆:
Polyclonal
特异性:
PKA R2 Antibody detects endogenous levels of total PKA R2.
RRID:
AB_2835474
引用格式: Affinity Biosciences Cat# DF3091, RRID:AB_2835474.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cAMP dependent protein kinase regulatory subunit alpha 2; cAMP dependent protein kinase regulatory subunit RII alpha; cAMP dependent protein kinase type II alpha regulatory chain; cAMP dependent protein kinase type II alpha regulatory subunit; cAMP-dependent protein kinase type II-alpha regulatory subunit; KAP2; KAP2_HUMAN; MGC3606; PKR 2; PKR2; PRKA R2; PRKAR 2; PRKAR2; PRKAR2A; Protein kinase A RII alpha subunit; Protein kinase cAMP dependent regulatory type II alpha;

抗原和靶标

免疫原:
Uniprot:

P13861(KAP2_HUMAN) >>Visit HPA database.

基因/基因ID:
PRKAR2A(5576)
表达:
P13861 KAP2_HUMAN:

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

序列:
MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
89
Bovine
78
Rabbit
63
Pig
0
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P13861 作为底物

Site PTM Type Enzyme
S2 Acetylation
S48 Phosphorylation
T54 Phosphorylation
S58 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation
S92 Phosphorylation
S99 Phosphorylation
C101 S-Nitrosylation
T104 Phosphorylation
Y105 Phosphorylation
T114 Phosphorylation
K122 Ubiquitination
K135 Ubiquitination
K161 Ubiquitination
Y177 Phosphorylation
K190 Ubiquitination
S196 Phosphorylation
Y200 Phosphorylation
S254 Phosphorylation
K263 Ubiquitination
S268 Phosphorylation
K272 Ubiquitination
K280 Ubiquitination
K315 Methylation
K318 Acetylation
K318 Ubiquitination
K332 Ubiquitination
T343 Phosphorylation
K345 Ubiquitination
S350 Phosphorylation
Y352 Phosphorylation
S383 Phosphorylation
Y385 Phosphorylation
S395 Phosphorylation
S396 Phosphorylation

翻译修饰 - P13861 作为激酶

Substrate Site Source
P13861-1 (PRKAR2A) S99 Uniprot

研究背景

功能:

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

翻译修饰:

Phosphorylated by the activated catalytic chain.

细胞定位:

Cytoplasm. Cell membrane.
Note: Colocalizes with PJA2 in the cytoplasm and the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

亚基结构:

The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3. Interacts with the phosphorylated form of PJA2. Interacts with MYRIP. This interaction may link PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release (By similarity). Forms a complex composed of PRKAR2A, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity. Interacts with ADCY8; inhibits adenylate cyclase activity through PKA phosphorylation.

蛋白家族:

Belongs to the cAMP-dependent kinase regulatory chain family.

研究领域

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.