产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3109, RRID:AB_2835486.
展开/折叠
Activating transcription factor 1; ATF 1; atf1; ATF1 EWS fusion gene; ATF1 FUS fusion gene; ATF1_HUMAN; cAMP dependent transcription factor 1; cAMP-dependent transcription factor ATF-1; Cyclic AMP dependent transcription factor ATF 1; Cyclic AMP dependent transcription factor ATF1; Cyclic AMP-dependent transcription factor ATF-1; EWS AFT1; FUS ATF 1; FUS/ATF 1; Protein TREB36; RNA binding protein activating transcription factor 1 fusion protein; TREB 36; TREB36; TREB36 protein;
抗原和靶标
- P18846 ATF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P18846 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | P68400 (CSNK2A1) , P48729 (CSNK1A1) | Uniprot |
| S38 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S41 | Phosphorylation | P48729 (CSNK1A1) , P68400 (CSNK2A1) | Uniprot |
| S44 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S47 | Phosphorylation | P48729 (CSNK1A1) , P68400 (CSNK2A1) | Uniprot |
| S50 | Phosphorylation | P68400 (CSNK2A1) , P48729 (CSNK1A1) | Uniprot |
| S51 | Phosphorylation | P48729 (CSNK1A1) , P68400 (CSNK2A1) | Uniprot |
| S63 | Phosphorylation | O75676 (RPS6KA4) , Q00526 (CDK3) , Q14012 (CAMK1) , O75582 (RPS6KA5) , P68400 (CSNK2A1) , P51812 (RPS6KA3) | Uniprot |
| Y64 | Phosphorylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| S189 | O-Glycosylation | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Q9H2X6 (HIPK2) | Uniprot |
| T209 | Phosphorylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| R244 | Methylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot |
研究背景
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.
Nucleus.
Binds DNA as a dimer. Interacts with HIPK2 and CDK3.
Belongs to the bZIP family. ATF subfamily.
研究领域
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
限制条款
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