(1)
(3)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3112, RRID:AB_2835489.
展开/折叠
Brain form hexokinase; Hexokinase 1; Hexokinase type I; HK I; HK1 ta; HK1 tb; HK1 tc; HKI; HXK1;
抗原和靶标
Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific (PubMed:10978502). Isoform 4: Testis-specific (PubMed:10978502).
- P19367 HXK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAVVKMLPSFVRRTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGILITWTKGFKATDCVGHDVVTLLRDAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVEMVEGDQGQMCINMEWGAFGDNGCLDDIRTHYDRLVDEYSLNAGKQRYEKMISGMYLGEIVRNILIDFTKKGFLFRGQISETLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTEASS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P19367 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| Y25 | Phosphorylation | Uniprot | |
| Y27 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K77 | Acetylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K176 | Methylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| T403 | Phosphorylation | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| T419 | Phosphorylation | Uniprot | |
| Y461 | Phosphorylation | Uniprot | |
| K489 | Acetylation | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| T503 | Phosphorylation | Uniprot | |
| K510 | Ubiquitination | Uniprot | |
| K544 | Acetylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| Y686 | Phosphorylation | Uniprot | |
| R727 | Methylation | Uniprot | |
| Y732 | Phosphorylation | P12931 (SRC) | Uniprot |
| S733 | Phosphorylation | Uniprot | |
| K738 | Ubiquitination | Uniprot | |
| Y749 | Phosphorylation | Uniprot | |
| K763 | Acetylation | Uniprot | |
| K763 | Ubiquitination | Uniprot | |
| K777 | Ubiquitination | Uniprot | |
| S792 | Phosphorylation | Uniprot | |
| C813 | S-Nitrosylation | Uniprot | |
| T821 | Phosphorylation | Uniprot | |
| C823 | S-Nitrosylation | Uniprot | |
| S827 | Phosphorylation | Uniprot | |
| R849 | Methylation | Uniprot | |
| K880 | Ubiquitination | Uniprot | |
| K885 | Ubiquitination | Uniprot | |
| S893 | Phosphorylation | Uniprot | |
| T913 | Phosphorylation | Uniprot |
研究背景
Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome.
Mitochondrion outer membrane>Peripheral membrane protein. Cytoplasm>Cytosol.
Note: The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331).
Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific. Isoform 4: Testis-specific.
Monomer. Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with VDAC1. The HK1-VDAC1 complex interacts with ATF2. Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity).
The N- and C-terminal halves of this hexokinase contain a hexokinase domain (PubMed:9493266, PubMed:9735292, PubMed:10574795). The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus (PubMed:9493266, PubMed:9735292, PubMed:10574795). Each domain can bind a single D-glucose and D-glucose 6-phosphate molecule (PubMed:9493266).
Belongs to the hexokinase family.
研究领域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.
· Metabolism > Carbohydrate metabolism > Galactose metabolism.
· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.
· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.
· Metabolism > Biosynthesis of other secondary metabolites > Neomycin, kanamycin and gentamicin biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.
文献引用
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