产品: TFE3 抗体
货号: DF3114
描述: Rabbit polyclonal antibody to TFE3
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 62 KD; 62kD(Calculated).
蛋白号: P19532
RRID: AB_2835491

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
TFE3 Antibody detects endogenous levels of total TFE3.
RRID:
AB_2835491
引用格式: Affinity Biosciences Cat# DF3114, RRID:AB_2835491.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bHLH e33; bHLHe33; Class E basic helix-loop-helix protein 33; RCCP 2; RCCP2; RCCX1; Renal cell carcinoma, papillary; Tcfe 3; Tcfe3; TFE 3; Tfe3; TFE3_HUMAN; TFEA; Transcription factor binding to IGHM enhancer 3; Transcription factor E family, member A; Transcription factor E3; Transcription factor for IgH enhancer; Transcription factor for immunoglobulin heavy chain enhancer 3;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P19532 TFE3_HUMAN:

Ubiquitous in fetal and adult tissues.

序列:
MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQPLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLPVPPTPGLLSLATTSASDSLKPEQLDIEEEGRPGAATFHVGGGPAQNAPHQQPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILMEEEEGVVGGLSGGALSPLRAASDPLLSSVSPAVSKASSRRSSFSMEEES

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P19532 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
K68 Sumoylation
T85 Phosphorylation
S101 Phosphorylation
S109 Phosphorylation
S112 Phosphorylation
R122 Methylation
S149 Phosphorylation
K178 Methylation
K200 Ubiquitination
Y202 Phosphorylation
T217 Phosphorylation
S246 Phosphorylation
S321 Phosphorylation
K330 Sumoylation
S334 Phosphorylation
K339 Ubiquitination
R365 Methylation
K385 Ubiquitination
K401 Acetylation
S542 Phosphorylation
S548 Phosphorylation
S554 Phosphorylation
S556 Phosphorylation
S560 Phosphorylation
S563 Phosphorylation
S564 Phosphorylation
S567 Phosphorylation
S568 Phosphorylation
S570 Phosphorylation
S575 Phosphorylation

研究背景

功能:

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') (By similarity). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (By similarity). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1.

翻译修饰:

Sumoylated; does not affect dimerization with MITF.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous in fetal and adult tissues.

亚基结构:

Homodimer and heterodimer; with TFEB or MITF.

蛋白家族:

Belongs to the MiT/TFE family.

研究领域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

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