| 产品: | p73 抗体 |
| 货号: | AF0322 |
| 描述: | Rabbit polyclonal antibody to p73 |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken |
| 分子量: | 80kDa; 70kD(Calculated). |
| 蛋白号: | O15350 |
| RRID: | AB_2833485 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0322, RRID:AB_2833485.
展开/折叠
Delta N p73; DNp73; p53 like transcription factor; p53 related protein; P73; TP73;
抗原和靶标
Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
- O15350 P73_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSAKNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQLLRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15350 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T14 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | P53350 (PLK1) | Uniprot |
| Y28 | Phosphorylation | P08631 (HCK) | Uniprot |
| S47 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S48 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| T86 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| Y99 | Phosphorylation | P00519 (ABL1) , A0A173G4P4 (Abl fusion) | Uniprot |
| T167 | Phosphorylation | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | O14965 (AURKA) | Uniprot |
| S289 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| K321 | Acetylation | Uniprot | |
| K327 | Acetylation | Uniprot | |
| K331 | Acetylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| S388 | Phosphorylation | P05771-2 (PRKCB) , P17252 (PRKCA) | Uniprot |
| S426 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S471 | Phosphorylation | Uniprot | |
| T568 | Phosphorylation | Uniprot | |
| K627 | Sumoylation | Uniprot |
研究背景
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function.
Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome.
Nucleus. Cytoplasm.
Note: Accumulates in the nucleus in response to DNA damage.
Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
Found in a complex with p53/TP53 and CABLES1. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Interacts with HECW2. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Isoform Beta interacts with WWOX. Interacts (via SAM domain) with FBXO45 (via B30.2/SPRY domain). Interacts with YAP1 (phosphorylated form). Interacts with HCK (via SH3 domain); this inhibits TP73 activity and degradation.
(Microbial infection) Interacts with Epstein-Barr virus protein EBNA6; this interaction inhibits TP73-mediated apoptotic pathway.
Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL1 tyrosine kinase SH3 domain.
The PPxY motif mediates interaction with WWOX.
Belongs to the p53 family.
研究领域
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Measles.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.