| 产品: | NFYB 抗体 |
| 货号: | DF3131 |
| 描述: | Rabbit polyclonal antibody to NFYB |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 29 KD; 23kD(Calculated). |
| 蛋白号: | P25208 |
| RRID: | AB_2835508 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3131, RRID:AB_2835508.
展开/折叠
CAAT box DNA binding protein subunit B; CAAT box DNA-binding protein subunit B; CBF A; CBF B; CCAAT binding transcription factor subunit A; HAP 3; HAP3; NF Y protein chain B; NF YB; NF-YB; NFYB; NFYB_HUMAN; Nuclear transcription factor Y beta; Nuclear transcription factor Y subunit B; Nuclear transcription factor Y subunit beta; Transcription factor NF Y B subunit;
抗原和靶标
- P25208 NFYB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P25208 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Phosphorylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot |
研究背景
Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Monoubiquitination at Lys-140 plays an important role in transcriptional activation by allowing the deposition of histone H3 methylations as well as histone H2B monoubiquitination at 'Lys-121'.
Nucleus.
Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. Interacts with C1QBP.
Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.
Belongs to the NFYB/HAP3 subunit family.
研究领域
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
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