MARK3 Antibody - #DF3135

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产品: MARK3 抗体
货号: DF3135
描述: Rabbit polyclonal antibody to MARK3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Rabbit, Dog, Chicken
分子量: 85 KD; 84kD(Calculated).
蛋白号: P27448
RRID: AB_2835512

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
MARK3 Antibody detects endogenous levels of total MARK3.
RRID:
AB_2835512
引用格式: Affinity Biosciences Cat# DF3135, RRID:AB_2835512.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C-TAK1; Cdc25C associated protein kinase 1; Cdc25C-associated protein kinase 1; cTAK1; ELKL motif kinase 2; EMK-2; Emk2; ETK 1; KIAA4230; KP78; MAP microtubule affinity regulating kinase 3; MAP/microtubule affinity-regulating kinase 3; Mark3; MARK3_HUMAN; Par 1a; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Serine threonine protein kinase p78; Serine/threonine-protein kinase p78; SerThr protein kinase PAR 1;

抗原和靶标

免疫原:
Uniprot:

P27448(MARK3_HUMAN) >>Visit HPA database.

基因/基因ID:
MARK3(4140)
表达:
P27448 MARK3_HUMAN:

Ubiquitous.

序列:
MSTRTPLPTVNERDTENHTSHGDGRQEVTSRTSRSGARCRNSIASCADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSSELDASDSSSSSNLSLAKVRPSSDLNNSTGQSPHHKVQRSVSSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPNKADIPERKKSSTVPSSNTASGGMTRRNTYVCSERTTADRHSVIQNGKENSTIPDQRTPVASTHSISSAATPDRIRFPRGTASRSTFHGQPRERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRRNMSFRFIKRLPTEYERNGRYEGSSRNVSAEQKDENKEAKPRSLRFTWSMKTTSSMDPGDMMREIRKVLDANNCDYEQRERFLLFCVHGDGHAENLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIANELKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Dog
100
Chicken
100
Rabbit
100
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P27448 作为底物

Site PTM Type Enzyme
T3 Phosphorylation
T5 Phosphorylation
S42 Phosphorylation
S45 Phosphorylation
T61 Phosphorylation
K64 Ubiquitination
K85 Ubiquitination
K89 Ubiquitination
T90 Phosphorylation P11309 (PIM1)
T95 Phosphorylation P11309 (PIM1)
S96 Phosphorylation P11309 (PIM1)
K180 Ubiquitination
T211 Phosphorylation Q15831 (STK11)
S215 Phosphorylation Q15831 (STK11)
K227 Ubiquitination
S238 Phosphorylation
K294 Ubiquitination
K303 Ubiquitination
K317 Ubiquitination
K330 Ubiquitination
Y340 Phosphorylation
S341 Phosphorylation
K367 Ubiquitination
S374 Phosphorylation
S376 Phosphorylation
S377 Phosphorylation
S378 Phosphorylation
S379 Phosphorylation
S380 Phosphorylation
S383 Phosphorylation
S396 Phosphorylation
T397 Phosphorylation
S400 Phosphorylation
Y418 Phosphorylation
S419 Phosphorylation
Y432 Phosphorylation
T438 Phosphorylation
S455 Phosphorylation
S456 Phosphorylation
S458 Phosphorylation
S469 Phosphorylation
S476 Phosphorylation
S489 Phosphorylation
S490 Phosphorylation
T491 Phosphorylation
S494 Phosphorylation
S499 Phosphorylation
T503 Phosphorylation
T507 Phosphorylation P17612 (PRKACA)
Y508 Phosphorylation
S511 Phosphorylation
S520 Phosphorylation
T530 Phosphorylation
T536 Phosphorylation
S540 Phosphorylation
T541 Phosphorylation
S543 Phosphorylation
S545 Phosphorylation
S546 Phosphorylation
T549 Phosphorylation
S563 Phosphorylation
T564 Phosphorylation P41743 (PRKCI) , Q05513 (PRKCZ)
T574 Phosphorylation
T576 Phosphorylation
Y577 Phosphorylation
S583 Phosphorylation
S585 Phosphorylation
S587 Phosphorylation
T591 Phosphorylation
S594 Phosphorylation
T596 Phosphorylation
S598 Phosphorylation
R599 Methylation
S601 Phosphorylation
S606 Phosphorylation
S610 Phosphorylation
K611 Ubiquitination
S618 Phosphorylation
S643 Phosphorylation
S657 Phosphorylation
K681 Ubiquitination
Y690 Phosphorylation
S724 Phosphorylation
K741 Ubiquitination
S745 Phosphorylation

翻译修饰 - P27448 作为激酶

Substrate Site Source
P27448-3 (MARK3) S598 Uniprot
P27448-3 (MARK3) S601 Uniprot
P27448-3 (MARK3) T602 Uniprot
P27448-3 (MARK3) S606 Uniprot
P30307 (CDC25C) S216 Uniprot
P41236 (PPP1R2) S72 Uniprot
Q8WUI4 (HDAC7) S155 Uniprot

研究背景

功能:

Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.

翻译修饰:

Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity.

细胞定位:

Cell membrane>Peripheral membrane protein. Cell projection>Dendrite. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ.

蛋白家族:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

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