产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3135, RRID:AB_2835512.
展开/折叠
C-TAK1; Cdc25C associated protein kinase 1; Cdc25C-associated protein kinase 1; cTAK1; ELKL motif kinase 2; EMK-2; Emk2; ETK 1; KIAA4230; KP78; MAP microtubule affinity regulating kinase 3; MAP/microtubule affinity-regulating kinase 3; Mark3; MARK3_HUMAN; Par 1a; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Serine threonine protein kinase p78; Serine/threonine-protein kinase p78; SerThr protein kinase PAR 1;
抗原和靶标
- P27448 MARK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTRTPLPTVNERDTENHTSHGDGRQEVTSRTSRSGARCRNSIASCADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSSELDASDSSSSSNLSLAKVRPSSDLNNSTGQSPHHKVQRSVSSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPNKADIPERKKSSTVPSSNTASGGMTRRNTYVCSERTTADRHSVIQNGKENSTIPDQRTPVASTHSISSAATPDRIRFPRGTASRSTFHGQPRERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRRNMSFRFIKRLPTEYERNGRYEGSSRNVSAEQKDENKEAKPRSLRFTWSMKTTSSMDPGDMMREIRKVLDANNCDYEQRERFLLFCVHGDGHAENLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIANELKL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P27448 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T3 | Phosphorylation | Uniprot | |
| T5 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| T90 | Phosphorylation | P11309 (PIM1) | Uniprot |
| T95 | Phosphorylation | P11309 (PIM1) | Uniprot |
| S96 | Phosphorylation | P11309 (PIM1) | Uniprot |
| K180 | Ubiquitination | Uniprot | |
| T211 | Phosphorylation | Q15831 (STK11) | Uniprot |
| S215 | Phosphorylation | Q15831 (STK11) | Uniprot |
| K227 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| Y340 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| Y418 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| Y432 | Phosphorylation | Uniprot | |
| T438 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| T491 | Phosphorylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| T503 | Phosphorylation | Uniprot | |
| T507 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| Y508 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| T530 | Phosphorylation | Uniprot | |
| T536 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| T541 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| T549 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| T564 | Phosphorylation | P41743 (PRKCI) , Q05513 (PRKCZ) | Uniprot |
| T574 | Phosphorylation | Uniprot | |
| T576 | Phosphorylation | Uniprot | |
| Y577 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| S587 | Phosphorylation | Uniprot | |
| T591 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| T596 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| R599 | Methylation | Uniprot | |
| S601 | Phosphorylation | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| S643 | Phosphorylation | Uniprot | |
| S657 | Phosphorylation | Uniprot | |
| K681 | Ubiquitination | Uniprot | |
| Y690 | Phosphorylation | Uniprot | |
| S724 | Phosphorylation | Uniprot | |
| K741 | Ubiquitination | Uniprot | |
| S745 | Phosphorylation | Uniprot |
翻译修饰 - P27448 作为激酶
研究背景
Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.
Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity.
Cell membrane>Peripheral membrane protein. Cell projection>Dendrite. Cytoplasm.
Ubiquitous.
Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
限制条款
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