| 产品: | ARNT 抗体 |
| 货号: | DF3136 |
| 描述: | Rabbit polyclonal antibody to ARNT |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 87 KD; 87kD(Calculated). |
| 蛋白号: | P27540 |
| RRID: | AB_2835513 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3136, RRID:AB_2835513.
展开/折叠
arnT; ARNT protein; ARNT_HUMAN; Aryl hydrocarbon receptor nuclear translocator; bHLHe2; Class E basic helix-loop-helix protein 2; Dioxin receptor; Dioxin receptor nuclear translocator; Drnt; HIF 1 beta; HIF 1beta; HIF-1-beta; HIF1-beta; HIF1B; HIF1beta; Hypoxia Inducible Factor 1; Hypoxia inducible factor 1 beta; Hypoxia-inducible factor 1-beta; nuclear translocator; Tango;
抗原和靶标
- P27540 ARNT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSNVCQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKGQVLSVMFRFRSKNQEWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPTLSNTIQRPQLGPTANLPLEMGSGQLAPRQQQQQTELDMVPGRDGLASYNHSQVVQPVTTTGPEHSKPLEKSDGLFAQDRDPRFSEIYHNINADQSKGISSSTVPATQQLFSQGNTFPPTPRPAENFRNSGLAPPVTIVQPSASAGQMLAQISRHSNPTQGATPTWTPTTRSGFSAQQVATQATAKTRTSQFGVGSFQTPSSFSSMSLPGAPTASPGAAAYPSLTNRGSNFAPETGQTAGQFQTRTAEGVGVWPQWQGQQPHHRSSSSEQHVQQPPAQQPGQPEVFQEMLSMLGDQSNSYNNEEFPDLTMFPPFSE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P27540 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| K58 | Acetylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S82 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| R143 | Methylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| K544 | Ubiquitination | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| Y561 | Phosphorylation | Uniprot | |
| T593 | Phosphorylation | Uniprot | |
| T636 | Phosphorylation | Uniprot | |
| T638 | Phosphorylation | Uniprot | |
| T640 | Phosphorylation | Uniprot | |
| S669 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot |
研究背景
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.
Nucleus.
Monomer. Homodimer only upon binding to a DNA (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Interacts with TACC3 (By similarity). Interacts with HIF1A, EPAS1, NPAS1 and NPAS3; forms a heterodimer that binds core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Forms a heterodimer with AHRR, as well as with other bHLH proteins (Probable). Interacts with NOCA7. Interacts with TACC3 (By similarity). Interacts with AHR; the heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription. Interacts with SIM1 and NPAS4 (By similarity).
研究领域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Chemical carcinogenesis.
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
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