(3)
(6)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3139, RRID:AB_2835516.
展开/折叠
Autoantigen RO; CALR; CALR protein; CALR_HUMAN; Calregulin; Calreticulin; cC1qR; CRP55; CRT; CRTC; Endoplasmic reticulum resident protein 60; Epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; HEL S 99n; RO; Sicca syndrome antigen A (autoantigen Ro; calreticulin); Sicca syndrome antigen A; SSA;
抗原和靶标
- P27797 CALR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLLSVPLLLGLLGLAVAEPAVYFKEQFLDGDGWTSRWIESKHKSDFGKFVLSSGKFYGDEEKDKGLQTSQDARFYALSASFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDASKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P27797 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y22 | Phosphorylation | Uniprot | |
| K24 | Sumoylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| Y57 | Phosphorylation | Uniprot | |
| K62 | Acetylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K64 | Acetylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| R73 | Methylation | Uniprot | |
| Y75 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| C105 | S-Nitrosylation | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| K111 | Methylation | Uniprot | |
| K142 | Acetylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K153 | Acetylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K159 | Acetylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| K206 | Acetylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K207 | Acetylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K209 | Acetylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| R222 | Methylation | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K238 | Methylation | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| Y285 | Phosphorylation | Uniprot | |
| Y299 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| T333 | Phosphorylation | Uniprot | |
| N344 | N-Glycosylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| R366 | Methylation | Uniprot |
研究背景
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).
Endoplasmic reticulum lumen. Cytoplasm>Cytosol. Secreted>Extracellular space>Extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen.
Note: Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038). Associated with the lytic granules in the cytolytic T-lymphocytes.
Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 and SPACA9 (By similarity). Interacts with TRIM21. Interacts with NR3C1. Interacts with PPIB. Interacts (via P-domain) with PDIA5. Interacts with GABARAP. Interacts with HLA-E-B2M and HLA-G-B2M complexes. Interacts with HLA-F. Interacts with CLCC1.
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
Belongs to the calreticulin family.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
文献引用
Application: IF/ICC Species: Mouse Sample: 4T1 cells
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