PPIF Antibody - #DF3147

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产品: PPIF 抗体
货号: DF3147
描述: Rabbit polyclonal antibody to PPIF
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: P30405
RRID: AB_2835524

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PPIF Antibody detects endogenous levels of total PPIF.
RRID:
AB_2835524
引用格式: Affinity Biosciences Cat# DF3147, RRID:AB_2835524.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cyclophilin 3; cyclophilin D; Cyclophilin F; Cyp D; CyP M; CYP3; CypD; hCyP3; mitochondrial; Mitochondrial cyclophilin; Peptidyl prolyl cis trans isomerase, mitochondral; Peptidyl-prolyl cis-trans isomerase F; Peptidyl-prolyl cis-trans isomerase F, mitochondrial; Peptidylprolyl isomerase F (cyclophilin F); Peptidylprolyl isomerase F; PPIase; PPIase F; Ppif; PPIF_HUMAN; Rotamase; Rotamase F;

抗原和靶标

免疫原:

A synthesized peptide derived from human PPIF, corresponding to a region within the internal amino acids.

基因/基因ID:
PPIF(10105)

研究领域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

文献引用

1). Emodin targets mitochondrial cyclophilin D to induce apoptosis in HepG2 cells. BIOMEDICINE & PHARMACOTHERAPY, 2017 (PubMed: 28363167) [IF=6.9]

Application: WB    Species: human    Sample:

Fig. 3. Effects of ROS and ERK on CypD expression. Cells were treated with emodin in the absence or presence of 5mM CsA or 10 ng/mL EGF for 48 h, or 5 mM NAC for 6 h. Protein expression was determined by western blots. (A) CypD expression in the absence or presence of CsA. (B) p-ERK expression induced by emodin. (C) CypD expression in the absence or presence of EGF. (D) CypD expression in the absence or presence of NAC. (E) Determination of emodin-induced cytotoxicity activity in the absence or presence of EGF or NAC by MTT assay. Values are expressed as the means  SD; n = 3, * p < 0.05 versus control.
2). Chrysophanol localizes in mitochondria to promote cell death through upregulation of mitochondrial cyclophilin D in HepG2 cells. Chinese Herbal Medicines, 2021 (PubMed: 36117497) [IF=4.7]
3). Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice. Experimental and Therapeutic Medicine, 2022 (PubMed: 35069825) [IF=2.4]

Application: WB    Species: human    Sample: HK‑2 cells

Figure 2. | Expression levels of CypD, cytochrome c, eIF2α and GRP78 in HK‑2 cells following hypoxia/reoxygenation and DEX and 4‑PBA treatment. The left panel presents representative western blotting, and the right panel shows the quantified relative protein levels. *P<0.05 vs. control; #P<0.05 vs. model. CypD,cyclophilin D; eIF2α, eukaryotic translation initiation factor 2α; GRP78, glucose‑regulated protein 78.

Application: WB    Species: Human    Sample: HK-2 cells

Figure 2 Expression levels of CypD, cytochrome c, eIF2α and GRP78 in HK-2 cells following hypoxia/reoxygenation and DEX and 4-PBA treatment. The left panel presents representative western blotting, and the right panel shows the quantified relative protein levels. *P<0.05 vs. control; #P<0.05 vs. model. CypD, cyclophilin D; eIF2α, eukaryotic translation initiation factor 2α; GRP78, glucose-regulated protein 78.
4). Cyclophilin D Regulates Oxidative Stress and Apoptosis via Mitochondrial Permeability Transition Pore in Acute Acalculous Cholecystitis. CURRENT MOLECULAR MEDICINE, 2023 (PubMed: 36089783) [IF=2.2]
5). Bishonokiol A Induces Multiple Cell Death in Human Breast Cancer MCF-7 Cells. Asian Pacific Journal of Cancer Prevention, 2020 (PubMed: 32334473)

Application: WB    Species: Human    Sample: Breast Cancer MCF-7 Cells

Figure 3 BHNKA Induced Necroptosis in Human Breast Cancer MCF-7 Cells. (A), Morphological characteristics of cells treated with BHNKA (40 μM) for 24h by electron microscope. Red arrowheads indicated cell membrane integrity and swelling of cellular orgenelles. (B), Cell viability following treated with BHNKA (40 μM) with or without pre-treatment with necroptosis inhibitors (20 μM of Nec-1 and CsA) were measured by MTT assay. (C), Western blotting analyses were used to measure the expression of necroptosis-related proteins, such as RIP1, RIP3, MLKL, and CypD. * P < 0.05 compared with the control

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