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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3148, RRID:AB_2835525.
展开/折叠
Actin binding protein; CLABP; Clipin A; Clipin-A; CLIPINA; COR1A_HUMAN; CORO1A; Coronin 1; Coronin actin binding protein 1A; Coronin like protein A; Coronin like protein p57; Coronin-1A; Coronin-like protein A; Coronin-like protein p57; FLJ41407; HCORO1; MGC117380; OTTHUMP00000163017; p57; TACO; Tryptophan aspartate containing coat protein; Tryptophan aspartate-containing coat protein;
抗原和靶标
Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
- P31146 COR1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRQVVRSSKFRHVFGQPAKADQCYEDVRVSQTTWDSGFCAVNPKFVALICEASGGGAFLVLPLGKTGRVDKNAPTVCGHTAPVLDIAWCPHNDNVIASGSEDCTVMVWEIPDGGLMLPLREPVVTLEGHTKRVGIVAWHTTAQNVLLSAGCDNVIMVWDVGTGAAMLTLGPEVHPDTIYSVDWSRDGGLICTSCRDKRVRIIEPRKGTVVAEKDRPHEGTRPVRAVFVSEGKILTTGFSRMSERQVALWDTKHLEEPLSLQELDTSSGVLLPFFDPDTNIVYLCGKGDSSIRYFEITSEAPFLHYLSMFSSKESQRGMGYMPKRGLEVNKCEIARFYKLHERRCEPIAMTVPRKSDLFQEDLYPPTAGPDPALTAEEWLGGRDAGPLLISLKDGYVPPKSRELRVNRGLDTGRRRAAPEASGTPSSDAVSRLEEEMRKLQATVQELQKRLDRLEETVQAK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P31146 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K20 | Acetylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| Y25 | Phosphorylation | Uniprot | |
| T33 | Phosphorylation | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K233 | Acetylation | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| K313 | Acetylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K331 | Acetylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K339 | Acetylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| Y364 | Phosphorylation | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| S422 | Phosphorylation | Uniprot | |
| T424 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S426 | Phosphorylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| K449 | Acetylation | Uniprot | |
| K449 | Ubiquitination | Uniprot |
研究背景
May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
phosphorylation at Thr-412 by PKC strongly down-regulates the association with actin.
Polyubiquitinated by RNF128 with 'Lys-48'-linked chains, leading to proteasomal degradation.
Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Cytoplasmic vesicle>Phagosome membrane.
Note: In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification (By similarity).
Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
Binds actin.
Belongs to the WD repeat coronin family.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
文献引用
Application: WB Species: human Sample: AM-1 cells
Application: IHC Species: human Sample: oral mucosa tissues and ameloblastoma tissues
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