Gab2 Antibody - #AF0106

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(2)  
产品: Gab2 抗体
货号: AF0106
描述: Rabbit polyclonal antibody to Gab2
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 97kDa; 74kD(Calculated).
蛋白号: Q9UQC2
RRID: AB_2833282

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
Gab2 Antibody detects endogenous levels of total Gab2.
RRID:
AB_2833282
引用格式: Affinity Biosciences Cat# AF0106, RRID:AB_2833282.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GAB 2; Gab2; GAB2_HUMAN; Grb 2 associated binder 2; GRB 2 associated binding protein 2; Grb2 associated binder 2; GRB2 associated binder 2 pp100; GRB2 associated binding protein 2; GRB2-associated binder 2; GRB2-associated-binding protein 2; Growth factor receptor bound protein 2 associated protein 2; Growth factor receptor bound protein 2-associated protein 2; KIAA0571; p97; PH domain containing adaptor molecule p97; pp100;

抗原和靶标

免疫原:
Uniprot:

Q9UQC2(GAB2_HUMAN) >>Visit HPA database.

基因/基因ID:
GAB2(9846)
描述:
Gab2 an adaptor protein of the GRB2-associated binding protein family. Plays important roles in regulating the growth, differentiation, and function of many hematopoietic cell types.
序列:
MSGGGDVVCTGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGDPDVLEYYKNDHSKKPLRIINLNFCEQVDAGLTFNKKELQDSFVFDIKTSERTFYLVAETEEDMNKWVQSICQICGFNQAEESTDSLRNVSSAGHGPRSSPAELSSSSQHLLRERKSSAPSHSSQPTLFTFEPPVSNHMQPTLSTSAPQEYLYLHQCISRRAENARSASFSQGTRASFLMRSDTAVQKLAQGNGHCVNGISGQVHGFYSLPKPSRHNTEFRDSTYDLPRSLASHGHTKGSLTGSETDNEDVYTFKTPSNTLCREFGDLLVDNMDVPATPLSAYQIPRTFTLDKNHNAMTVATPGDSAIAPPPRPPKPSQAETPRWGSPQQRPPISENSRSVAATIPRRNTLPAMDNSRLHRASSCETYEYPQRGGESAGRSAESMSDGVGSFLPGKMIVGRSDSTNSEDNYVPMNPGSSTLLAMERAGDNSQSVYIPMSPGAHHFDSLGYPSTTLPVHRGPSRGSEIQPPPVNRNLKPDRKAKPTPLDLRNNTVIDELPFKSPITKSWSRANHTFNSSSSQYCRPISTQSITSTDSGDSEENYVPMQNPVSASPVPSGTNSPAPKKSTGSVDYLALDFQPSSPSPHRKPSTSSVTSDEKVDYVQVDKEKTQALQNTMQEWTDVRQSSEPSKGAKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Xenopus
88
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UQC2 作为底物

Site PTM Type Enzyme
Phosphorylation
S2 Phosphorylation
Y48 Phosphorylation
Y49 Phosphorylation
K56 Acetylation
S133 Phosphorylation
S140 Phosphorylation
S141 Phosphorylation
S146 Phosphorylation
S147 Phosphorylation
S148 Phosphorylation
S149 Phosphorylation
S159 Phosphorylation P31749 (AKT1)
S164 Phosphorylation
T171 Phosphorylation
S177 Phosphorylation
Y194 Phosphorylation
S208 Phosphorylation
S210 Phosphorylation
S218 Phosphorylation
S223 Phosphorylation
Y249 Phosphorylation
S250 Phosphorylation
S264 Phosphorylation
T265 Phosphorylation
Y266 Phosphorylation
T278 Phosphorylation
S281 Phosphorylation
S285 Phosphorylation
T287 Phosphorylation
Y293 Phosphorylation
T294 Phosphorylation
T319 Phosphorylation
S322 Phosphorylation
Y324 Phosphorylation
T331 Phosphorylation
S368 Phosphorylation
T385 Phosphorylation
T391 Phosphorylation
S404 Phosphorylation
S405 Phosphorylation
T408 Phosphorylation
Y409 Phosphorylation
Y411 Phosphorylation
S422 Phosphorylation
S425 Phosphorylation
S427 Phosphorylation
S448 Phosphorylation
Y452 Phosphorylation P08631 (HCK)
S459 Phosphorylation
S472 Phosphorylation
S474 Phosphorylation
Y476 Phosphorylation
S480 Phosphorylation
S488 Phosphorylation
Y491 Phosphorylation
K542 Methylation
S543 Phosphorylation
T546 Phosphorylation
S550 Phosphorylation
Y563 Phosphorylation
Y584 Phosphorylation
S611 Phosphorylation
Y614 Phosphorylation P43403 (ZAP70)
S622 Phosphorylation
S623 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S625 Phosphorylation
S631 Phosphorylation
T632 Phosphorylation
S633 Phosphorylation
S634 Phosphorylation
T636 Phosphorylation
S637 Phosphorylation
Y643 Phosphorylation O60674 (JAK2)
T651 Phosphorylation
T657 Phosphorylation

研究背景

功能:

Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

翻译修饰:

Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.

Dephosphorylated by PTPN11.

细胞定位:

Cytoplasm. Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with SHC1; may mediate interaction with receptors (By similarity). Interacts with SYK (By similarity). Interacts with PI-3 kinase. Interacts with GRB2 (via SH3 2 domain). Interacts (phosphorylated) with PTPN11. Interacts with TNFRSF11A (via cytoplasmic domain). Interacts (phosphorylated) with 14-3-3 family proteins SFN, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ and YWHAZ; prevents interaction with GRB2 and attenuates GAB2 signaling. Interacts with HCK.

蛋白家族:

The SH3-binding motifs mediate interaction with SHC1 and GRB2.

The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.

Belongs to the GAB family.

研究领域

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.