产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3157, RRID:AB_2835534.
展开/折叠
C2TA; C2TA_HUMAN; CIITA; CIITA IV; Class II major histocompatibility complex transactivator; Class II transactivator; MHC class II transactivator; MHC class II transactivator type III; MHC2TA; NLR family acid domain containing; NLRA; Nucleotide binding oligomerization domain leucine rich repeat and acid domain containing;
抗原和靶标
- P33076 C2TA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRCLAPRPAGSYLSEPQGSSQCATMELGPLEGGYLELLNSDADPLCLYHFYDQMDLAGEEEIELYSEPDTDTINCDQFSRLLCDMEGDEETREAYANIAELDQYVFQDSQLEGLSKDIFKHIGPDEVIGESMEMPAEVGQKSQKRPFPEELPADLKHWKPAEPPTVVTGSLLVRPVSDCSTLPCLPLPALFNQEPASGQMRLEKTDQIPMPFSSSSLSCLNLPEGPIQFVPTISTLPHGLWQISEAGTGVSSIFIYHGEVPQASQVPPPSGFTVHGLPTSPDRPGSTSPFAPSATDLPSMPEPALTSRANMTEHKTSPTQCPAAGEVSNKLPKWPEPVEQFYRSLQDTYGAEPAGPDGILVEVDLVQARLERSSSKSLERELATPDWAERQLAQGGLAEVLLAAKEHRRPRETRVIAVLGKAGQGKSYWAGAVSRAWACGRLPQYDFVFSVPCHCLNRPGDAYGLQDLLFSLGPQPLVAADEVFSHILKRPDRVLLILDGFEELEAQDGFLHSTCGPAPAEPCSLRGLLAGLFQKKLLRGCTLLLTARPRGRLVQSLSKADALFELSGFSMEQAQAYVMRYFESSGMTEHQDRALTLLRDRPLLLSHSHSPTLCRAVCQLSEALLELGEDAKLPSTLTGLYVGLLGRAALDSPPGALAELAKLAWELGRRHQSTLQEDQFPSADVRTWAMAKGLVQHPPRAAESELAFPSFLLQCFLGALWLALSGEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLIFQPPARCLGALLGPSAAASVDRKQKVLARYLKRLQPGTLRARQLLELLHCAHEAEEAGIWQHVVQELPGRLSFLGTRLTPPDAHVLGKALEAAGQDFSLDLRSTGICPSGLGSLVGLSCVTRFRAALSDTVALWESLQQHGETKLLQAAEEKFTIEPFKAKSLKDVEDLGKLVQTQRTRSSSEDTAGELPAVRDLKKLEFALGPVSGPQAFPKLVRILTAFSSLQHLDLDALSENKIGDEGVSQLSATFPQLKSLETLNLSQNNITDLGAYKLAEALPSLAASLLRLSLYNNCICDVGAESLARVLPDMVSLRVMDVQYNKFTAAGAQQLAASLRRCPHVETLAMWTPTIPFSVQEHLQQQDSRISLR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P33076 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K141 | Acetylation | Uniprot | |
| K144 | Acetylation | Uniprot | |
| S280 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S286 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S293 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S373 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S427 | Phosphorylation | Uniprot | |
| Y428 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| T546 | Phosphorylation | Uniprot | |
| S834 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T838 | Phosphorylation | Uniprot | |
| T947 | Phosphorylation | Uniprot | |
| K959 | Acetylation | Uniprot | |
| S1050 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S1073 | Phosphorylation | Uniprot |
研究背景
Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro).
Autophosphorylated, affecting interaction with TAF7.
Nucleus. Nucleus>PML body.
Note: Recruited to PML body by PML.
Interacts with ZXDA and ZXDC. Interacts with PML (isoform PML-2).
The acetyltransferase domain is necessary for activation of both class I and class II transcription.
The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.
研究领域
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
限制条款
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