产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3158, RRID:AB_2835535.
展开/折叠
betaCstF 64 variant 2; CF-1 64 kDa subunit; CF1 64 kDa subunit; Cleavage stimulation factor 64 kDa subunit; Cleavage stimulation factor subunit 2; Cleavage stimulation factor, 3' pre RNA, subunit 2, 64kDa; CSTF 2; CstF 64; CSTF 64 kDa subunit; CstF-64; CSTF2; CSTF2_HUMAN; MGC188397;
抗原和靶标
- P33240 CSTF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQMFELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHGAGPGSGSNVSMNQQNPQAPQAQSLGGMHVNGAPPLMQASMQGGVPAPGQMPAAVTGPGPGSLAPGGGMQAQVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPPHQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLDGRGGRDPRGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQVPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P33240 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S14 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| T118 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| R303 | Methylation | Uniprot | |
| R308 | Methylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| R319 | Methylation | Uniprot | |
| R330 | Methylation | Uniprot | |
| T333 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| R344 | Methylation | Uniprot | |
| R365 | Methylation | Uniprot | |
| R373 | Methylation | Uniprot | |
| R381 | Methylation | Uniprot | |
| R388 | Methylation | Uniprot | |
| R402 | Methylation | Uniprot | |
| R405 | Methylation | Uniprot | |
| R423 | Methylation | Uniprot | |
| R428 | Methylation | Uniprot | |
| R458 | Methylation | Uniprot | |
| R463 | Methylation | Uniprot | |
| R468 | Methylation | Uniprot | |
| R475 | Methylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| R499 | Methylation | Uniprot | |
| T507 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| T531 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K572 | Ubiquitination | Uniprot |
研究背景
One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).
Nucleus.
Note: Localized with DDX1 in cleavage bodies.
The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells. Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1.
研究领域
· Genetic Information Processing > Translation > mRNA surveillance pathway.
限制条款
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