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产品:	TBX1 抗体
货号:	AF0327
描述:	Rabbit polyclonal antibody to TBX1
应用:	WB IF/ICC
反应:	Human
分子量:	43kDa; 43kD(Calculated).
蛋白号:	O43435
RRID:	AB_2833490

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二抗
封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:3000, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human

克隆:

Polyclonal

特异性:

TBX1 Antibody detects endogenous levels of total TBX1.

RRID:

AB_2833490
引用格式: Affinity Biosciences Cat# AF0327, RRID:AB_2833490.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Brachyury; CAFS; CTHM; DGCR; DGS; DORV; T box; T box 1; T box 1 transcription factor; T box 1 transcription factor C; T box protein 1; T box transcription factor TBX 1; T box transcription factor TBX1; T-box protein 1; T-box transcription factor TBX1; TBX 1; TBX 1C; tbx1; TBX1_HUMAN; TBX1C; Testis specific T box protein; Testis-specific T-box protein; TGA; VCFS;

抗原和靶标

免疫原:

A synthesized peptide derived from human TBX1, corresponding to a region within C-terminal amino acids.

Uniprot:

O43435(TBX1_HUMAN) >>Visit HPA database.

基因/基因ID:

TBX1(6899)

描述:

TBX1 Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries. 3 isoforms of the human protein are produced by alternative splicing.

序列:

O43435 TBX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MHFSTVTRDMEAFTASSLSSLGAAGGFPGAASPGADPYGPREPPPPPPRYDPCAAAAPGAPGPPPPPHAYPFAPAAGAATSAAAEPEGPGASCAAAAKAPVKKNAKVAGVSVQLEMKALWDEFNQLGTEMIVTKAGRRMFPTFQVKLFGMDPMADYMLLMDFVPVDDKRYRYAFHSSSWLVAGKADPATPGRVHYHPDSPAKGAQWMKQIVSFDKLKLTNNLLDDNGHIILNSMHRYQPRFHVVYVDPRKDSEKYAEENFKTFVFEETRFTAVTAYQNHRITQLKIASNPFAKGFRDCDPEDWPRNHRPGALPLMSAFARSRNPVASPTQPSGTEKGGHVLKDKEVKAETSRNTPEREVELLRDAGGCVNLGLPCPAECQPFNTQGLVAGRTAGDRLC

翻译修饰 - O43435 作为底物

O43435

Site	PTM Type	Source
T142	Phosphorylation	Uniprot
K202	Ubiquitination	Uniprot
Y255	Phosphorylation	Uniprot
T282	Phosphorylation	Uniprot
S327	Phosphorylation	Uniprot

研究背景

O43435_TBX1_HUMAN

功能:

Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries (By similarity).

细胞定位:

Nucleus.

亚基结构:

Interacts with DSCR6.

文献引用

1). Establishment of an induced pluripotent stem cell line (SAHGMUi001-A) from a patient with Netherton syndrome carrying SPINK5 mutation. Stem Cell Research, 2021 (PubMed: 32416580) [IF=1.2]

2). Generation and characterization of an induced pluripotent stem cell line (ZSYYDNi001-A) from a patient with Duchenne muscular dystrophy carrying exon 51 deletion in the DMD gene. Stem Cell Research, 2021 (PubMed: 34619646) [IF=1.2]

Application: IF/ICC Species: Human Sample:

Fig. 1. Characterization of the induced pluripotent stem cell line.

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