产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3168, RRID:AB_2835544.
展开/折叠
FLJ36364; MGC47735; OTTHUMP00000159391; T cell factor 1; T cell specific transcription factor 1; T-cell factor 1; T-cell-specific transcription factor 1; TCF-1; TCF-7; TCF1; Tcf7; TCF7 transcription factor 7; TCF7_HUMAN; Transcription factor 7 (T cell specific HMG box); Transcription factor 7; Transcription factor-7, T-cell specific; Transcription factor-7, T-cell specific, 1;
抗原和靶标
Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
- P36402 TCF7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPQLDSGGGGAGGGDDLGAPDELLAFQDEGEEQDDKSRDSAAGPERDLAELKSSLVNESEGAAGGAGIPGVPGAGAGARGEAEALGREHAAQRLFPDKLPEPLEDGLKAPECTSGMYKETVYSAFNLLMHYPPPSGAGQHPQPQPPLHKANQPPHGVPQLSLYEHFNSPHPTPAPADISQKQVHRPLQTPDLSGFYSLTSGSMGQLPHTVSWFTHPSLMLGSGVPGHPAAIPHPAIVPPSGKQELQPFDRNLKTQAESKAEKEAKKPTIKKPLNAFMLYMKEMRAKVIAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKRRSREKHQESTTETNWPRELKDGNGQESLSMSSSSSPA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P36402 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K36 | Ubiquitination | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| Y117 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| Y163 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| Y318 | Phosphorylation | Uniprot | |
| Y319 | Phosphorylation | Uniprot | |
| Y333 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot |
研究背景
Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
Nucleus.
Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with TLE5, TLE1, TLE2, TLE3 and TLE4. Interacts with MLLT11.
Belongs to the TCF/LEF family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Endometrial cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Thyroid cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
· Organismal Systems > Endocrine system > Melanogenesis.
限制条款
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