BUD31 Antibody - #DF3175

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产品: BUD31 抗体
货号: DF3175
描述: Rabbit polyclonal antibody to BUD31
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 17 KD; 17kD(Calculated).
蛋白号: P41223
RRID: AB_2835551

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
BUD31 Antibody detects endogenous levels of total BUD31.
RRID:
AB_2835551
引用格式: Affinity Biosciences Cat# DF3175, RRID:AB_2835551.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Bud31; BUD31 homolog (S. cerevisiae); BUD31_HUMAN; Cwc14; EDG 2; EDG2; fSAP17; Functional spliceosome associated protein 17; G10; G10 maternal transcript homolog; Maternal G10 transcript; Protein BUD31 homolog; Protein EDG-2; Protein G10 homolog; YCR063W;

抗原和靶标

免疫原:
Uniprot:

P41223(BUD31_HUMAN) >>Visit HPA database.

基因/基因ID:
BUD31(8896)
表达:
P41223 BUD31_HUMAN:

Detected in epithelial and stromal cells in benign prostate hyperplasia tissue (at protein level).

序列:
MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
91
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P41223 作为底物

Site PTM Type Enzyme
K9 Ubiquitination
K28 Ubiquitination
K40 Ubiquitination
K42 Sumoylation
K42 Ubiquitination
K66 Ubiquitination
K80 Ubiquitination
K86 Ubiquitination
K91 Ubiquitination
K94 Ubiquitination
Y97 Phosphorylation
T115 Phosphorylation
K123 Ubiquitination
K125 Acetylation
K125 Sumoylation
K125 Ubiquitination
R140 Methylation

研究背景

功能:

Involved in the pre-mRNA splicing process. May play a role as regulator of AR transcriptional activity; may increase AR transcriptional activity.

细胞定位:

Nucleus.
Note: Detected in chromatin at the promoter of AR target genes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in epithelial and stromal cells in benign prostate hyperplasia tissue (at protein level).

亚基结构:

Identified in the spliceosome C complex. May interact with AR.

蛋白家族:

Contains a short sequence motif (Phe-Xaa-Xaa-Phe-Tyr) that can bind to AR and may modulate AR activity.

Belongs to the BUD31 (G10) family.

研究领域

· Genetic Information Processing > Transcription > Spliceosome.

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