产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0330, RRID:AB_2833492.
展开/折叠
1500031L16Rik; A kinase (PRKA) anchor protein 10; A kinase anchor protein 10; A kinase anchor protein 10 mitochondrial; A-kinase anchor protein 10; AKA10_HUMAN; akap 10; AKAP-10; AKAP10; B130049N18Rik; D AKAP 2; D-AKAP-2; dakap 2; dakap2; Dual specific A kinase anchoring protein 2; Dual specificity A kinase-anchoring protein 2; mgc9410; MGC9414|; Mitochondrial A kinase PPKA anchor protein 10; mitochondrial; prka 10; PRKA10; Protein A kinase anchoring protein 10; Protein A kinase anchoring protein 10 mitochondrial precursor; Protein kinase A anchoring protein 10; Protein kinase A-anchoring protein 10;
抗原和靶标
- O43572 AKA10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKSSLSKTLEQVLHDTIVLPYFIQFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQSSLAEPVSPSKKHETTASFLTDSLDKRLEDSGSAQLFMTHSEGIDLNNRTNSTQNHLLLSQECDSAHSLRLEMARAGTHQVSMETQESSSTLTVASRNSPASPLKELSGKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLTAPGSVGPPDESHPGSSDSSASQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRESEPEPDVRKSKGSMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDIMQQAQYDQPLEKSTKL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43572 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R2 | Methylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| R9 | Methylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| K55 | Methylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| T90 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| T272 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| Y442 | Phosphorylation | Uniprot | |
| T448 | Phosphorylation | Uniprot | |
| K559 | Ubiquitination | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| K637 | Ubiquitination | Uniprot | |
| K658 | Ubiquitination | Uniprot |
研究背景
Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase (By similarity).
Mitochondrion. Membrane. Cytoplasm.
Note: Predominantly mitochondrial but also membrane associated and cytoplasmic.
RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
限制条款
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