产品: TDG 抗体
货号: DF3267
描述: Rabbit polyclonal antibody to TDG
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 46 KD; 46kD(Calculated).
蛋白号: Q13569
RRID: AB_2835565

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
TDG Antibody detects endogenous levels of total TDG.
RRID:
AB_2835565
引用格式: Affinity Biosciences Cat# DF3267, RRID:AB_2835565.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C JUN leucine zipper interactive protein; C JUN leucine zipper interactive protein; C-JUN leucine zipper interactive protein JZA-3; E130317C12Rik; EC 3.2.2.29; G/T mismatch specific thymine DNA glycosylase; G/T mismatch specific thymine DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase; JZA 3; Jza1; T:G mismatch thymine glycosylase; Tdg; TDG_HUMAN; Thymine DNA glycosylase; Thymine-DNA glycosylase;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MEAENAGSYSLQQAQAFYTFPFQQLMAEAPNMAVVNEQQMPEEVPAPAPAQEPVQEAPKGRKRKPRTTEPKQPVEPKKPVESKKSGKSAKSKEKQEKITDTFKVKRKVDRFNGVSEAELLTKTLPDILTFNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYGIGFTNMVERTTPGSKDLSSKEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYVMPSSSARCAQFPRAQDKVHYYIKLKDLRDQLKGIERNMDVQEVQYTFDLQLAQEDAKKMAVKEEKYDPGYEAAYGGAYGENPCSSEPCGFSSNGLIESVELRGESAFSGIPNGQWMTQSFTDQIPSFSNHCGTQEQEEESHA

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
88
Rabbit
88
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13569 作为底物

Site PTM Type Enzyme
K71 Ubiquitination
S82 Phosphorylation
K83 Acetylation
K84 Acetylation
S85 Phosphorylation
K87 Acetylation
K92 Acetylation
K94 Acetylation
K97 Acetylation
K97 Ubiquitination
K103 Ubiquitination
K107 Ubiquitination
Y185 Phosphorylation
K201 Ubiquitination
K206 Ubiquitination
K218 Ubiquitination
K221 Ubiquitination
K232 Ubiquitination
S239 Phosphorylation
K246 Ubiquitination
K248 Sumoylation
K248 Ubiquitination
K258 Ubiquitination
K285 Ubiquitination
K300 Ubiquitination
K330 Sumoylation

研究背景

功能:

DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

翻译修饰:

Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer. Interacts with AICDA and GADD45A.

蛋白家族:

Belongs to the uracil-DNA glycosylase (UDG) superfamily. TDG/mug family.

研究领域

· Genetic Information Processing > Replication and repair > Base excision repair.

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