产品: AKAP8 抗体
货号: AF0333
描述: Rabbit polyclonal antibody to AKAP8
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Dog
分子量: 76kDa; 76kD(Calculated).
蛋白号: O43823
RRID: AB_2833493

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 50ul RMB¥ 1250 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
AKAP8 Antibody detects endogenous levels of total AKAP8.
RRID:
AB_2833493
引用格式: Affinity Biosciences Cat# AF0333, RRID:AB_2833493.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A kinase (PRKA) anchor protein 8; A kinase anchor protein 8; A kinase anchor protein 95; A kinase anchor protein 95 kDa; A kinase anchor protein 95kDa; A-kinase anchor protein 8; A-kinase anchor protein 95 kDa; AKAP 8; AKAP 95; AKAP-8; AKAP-95; Akap8; AKAP8_HUMAN; AKAP95; DKFZp586B1222;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O43823 AKAP8_HUMAN:

Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. Expressed in mature dendritic cells.

描述:
AKAP8 Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Belongs to the AKAP95 family. Binds to dimeric RII-alpha regulatory subunit of PKA during mitosis. Interacts (via C-terminus) with FIGN.
序列:
MDQGYGGYGAWSAGPANTQGAYGTGVASWQGYENYNYYGAQNTSVTTGATYSYGPASWEAAKANDGGLAAGAPAMHMASYGPEPCTDNSDSLIAKINQRLDMMSKEGGRGGSGGGGEGIQDRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O43823 作为底物

Site PTM Type Enzyme
Y51 Phosphorylation P12931 (SRC) , P07948 (LYN)
Y53 Phosphorylation P07948 (LYN) , P12931 (SRC)
Y80 Phosphorylation P07948 (LYN) , P12931 (SRC)
K95 Ubiquitination
R109 Methylation
S112 Phosphorylation
S136 Phosphorylation
Y146 Phosphorylation P07948 (LYN) , P12931 (SRC)
S149 Phosphorylation
Y150 Phosphorylation P07948 (LYN) , P12931 (SRC)
Y152 Phosphorylation P12931 (SRC) , P07948 (LYN)
Y154 Phosphorylation P07948 (LYN) , P12931 (SRC)
S165 Phosphorylation
Y170 Phosphorylation P12931 (SRC) , P07948 (LYN)
S182 Phosphorylation
R188 Methylation
R190 Methylation
R194 Methylation
R198 Methylation
S199 Phosphorylation
Y229 Phosphorylation
R233 Methylation
R242 Methylation
R277 Methylation
R281 Methylation
T301 Phosphorylation
K306 Ubiquitination
Y311 Phosphorylation P07948 (LYN) , P12931 (SRC)
K317 Ubiquitination
S323 Phosphorylation
S328 Phosphorylation
S339 Phosphorylation
C351 S-Nitrosylation
S353 Phosphorylation
S399 Phosphorylation
K407 Ubiquitination
T423 Phosphorylation
K424 Ubiquitination
Y436 Phosphorylation P07948 (LYN) , P12931 (SRC)
K465 Ubiquitination
K517 Ubiquitination
K524 Ubiquitination
K534 Acetylation
K538 Acetylation
Y539 Phosphorylation P12931 (SRC) , P07948 (LYN)
S644 Phosphorylation
T681 Phosphorylation
S685 Phosphorylation

研究背景

功能:

Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring. May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L. Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation. May be involved in regulation of DNA replication by acting as scaffold for MCM2. Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation. May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells. May act as a carrier protein of GJA1 for its transport to the nucleus. Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters. Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity).

翻译修饰:

Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases; multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes.

细胞定位:

Nucleus. Nucleus matrix. Nucleus>Nucleolus. Cytoplasm.
Note: Associated with the nuclear matrix in interphase and redistributes mostly to chromatin at mitosis. However, mitotic chromatin localization has been questioned. Upon nuclear reassembly at the end of mitosis, is sequestered into the daughter nuclei where it re-acquires an interphase distribution. Localized to the nucleolus in interphase. Colocalizes with GJA1 at the nuclear membrane specifically during cell cycle G1/S phase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. Expressed in mature dendritic cells.

亚基结构:

Binds to the PKA RII-alpha regulatory subunit PRKAR2A (phosphorylated at 'Thr-54') during mitosis. Interacts (via C-terminus) with FIGN (By similarity). Interacts with NCAPD2, CCND1, MCM2, RPS6KA1, PDE4A. Interacts with CCND3, CCNE1, DDX5, CASP3. Interacts with NFKB1; detetcted in the cytoplasm. Interacts with MYCBP; MYCBP is translocated to the nucleus and the interaction prevents the association of the PKA catalytic subunit leading to suppression of PKA activity (By similarity). Interacts with DPY30; mediating AKAP8 association with at least the MLL4/WBP7 HMT complex. Interacts with HDAC3; increased during mitosis. Interacts with GJA1; in the nucleus and in the nuclear membrane; the nuclear association increases with progress of cell cycle G1, S and G2 phase and decreases in M phase.

蛋白家族:

Belongs to the AKAP95 family.

文献引用

1). AKAP95 regulates ubiquitination and degradation of cyclin Ds/Es, influencing the G1/S transition of lung cancer cells. Molecular carcinogenesis, 2024 (PubMed: 38923703) [IF=4.6]

2). AKAP95 transports Cx43 into nucleus, regulating G1/S transition of A549 cells.. Research Square, 2020

Application: WB    Species: Human    Sample: A549 cells

AKAP95 bind to Cx43 and transfer it into nucleus during G1 phase in A549 cells.

3). Application of TEM: Dynamic changes of AKAP95-Cx43 complex during G1 phase of lung cancer cells. Research Square, 2020

Application: WB    Species: Human    Sample: A549 and Beas-2B cells

Figure1 TEM images show that AKAP95 carries Cx43 into the nucleus through nuclear pore by binding with it during G1 phase.

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