| 产品: | EPHB4 抗体 |
| 货号: | DF3215 |
| 描述: | Rabbit polyclonal antibody to EPHB4 |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 108~130kD; 108kD(Calculated). |
| 蛋白号: | P54760 |
| RRID: | AB_2835595 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3215, RRID:AB_2835595.
展开/折叠
Ephb4; EPHB4_HUMAN; Ephrin receptor EphB4; Ephrin type-B receptor 4; Hepatoma Transmembrane Kinase; HTK; MYK 1; MYK1; TYRO 11; TYRO11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11;
抗原和靶标
Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.
- P54760 EPHB4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLALIAGTAVVGVVLVLVVIVVAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P54760 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T34 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| T544 | Phosphorylation | Uniprot | |
| Y574 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| Y581 | Phosphorylation | Uniprot | |
| T587 | Phosphorylation | Uniprot | |
| Y590 | Phosphorylation | Uniprot | |
| T595 | Phosphorylation | Uniprot | |
| Y596 | Phosphorylation | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| Y614 | Phosphorylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| T713 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| T773 | Phosphorylation | Uniprot | |
| Y774 | Phosphorylation | Uniprot | |
| T775 | Phosphorylation | Uniprot | |
| S776 | Phosphorylation | Uniprot | |
| S777 | Phosphorylation | Uniprot | |
| K781 | Ubiquitination | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| K885 | Ubiquitination | Uniprot | |
| Y906 | Phosphorylation | Uniprot | |
| S911 | Phosphorylation | Uniprot | |
| Y924 | Phosphorylation | Uniprot | |
| S943 | Phosphorylation | Uniprot | |
| T953 | Phosphorylation | Uniprot | |
| K960 | Ubiquitination | Uniprot | |
| S964 | Phosphorylation | Uniprot | |
| K973 | Ubiquitination | Uniprot | |
| T976 | Phosphorylation | Uniprot | |
| Y987 | Phosphorylation | Uniprot |
翻译修饰 - P54760 作为激酶
研究背景
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.
Phosphorylated; autophosphorylation is stimulated by EFNB2.
Cell membrane>Single-pass type I membrane protein.
Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts with RASA1; the interaction depends on EPHB4 tyrosine-phosphorylation.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
研究领域
· Organismal Systems > Development > Axon guidance. (View pathway)
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