CKS1 Antibody - #DF3221

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产品: CKS1 抗体
货号: DF3221
描述: Rabbit polyclonal antibody to CKS1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 9 KD; 10kD(Calculated).
蛋白号: P61024
RRID: AB_2835601

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
CKS1 Antibody detects endogenous levels of total CKS1.
RRID:
AB_2835601
引用格式: Affinity Biosciences Cat# DF3221, RRID:AB_2835601.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDC2 associated protein CKS1; CDC28 protein kinase 1; CDC28 protein kinase 1B; CDC28 protein kinase regulatory subunit 1B; Cell division control protein CKS1; CKS 1; CKS-1; CKS1; CKS1_HUMAN; Cks1b; Ckshs1; Cyclin dependent kinases regulatory subunit 1; Cyclin-dependent kinases regulatory subunit 1; NB4 apoptosis/differentiation related protein; PNAS 143; PNAS 16; PNAS 18;

抗原和靶标

免疫原:
Uniprot:

P61024(CKS1_HUMAN) >>Visit HPA database.

基因/基因ID:
CKS1B(1163)
序列:
MSHKQIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTHLMSESEWRNLGVQQSQGWVHYMIHEPEPHILLFRRPLPKKPKK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
91
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P61024 作为底物

Site PTM Type Enzyme
S2 Acetylation
K4 Ubiquitination
Y7 Phosphorylation
Y8 Phosphorylation
S9 Phosphorylation
K11 Ubiquitination
Y12 Phosphorylation
Y19 Phosphorylation
K26 Ubiquitination
K30 Ubiquitination
K34 Ubiquitination
S39 Phosphorylation
S41 Phosphorylation
S51 Phosphorylation
Y57 Phosphorylation

研究背景

功能:

Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.

亚基结构:

Forms a homohexamer that can probably bind six kinase subunits.

蛋白家族:

Belongs to the CKS family.

研究领域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

文献引用

1). Identification of CKS1B as a prognostic indicator and a predictive marker for immunotherapy in pancreatic cancer. Frontiers in Immunology, 2022 (PubMed: 36405738) [IF=5.7]

Application: WB    Species: Human    Sample: PC cells

Figure 8 Knocking down CKS1B inhibits autophagy and STAT3/PD-L1 signaling in PC cells. (A, B) mRNA and protein expression of CKS1B in cells transfected with shCKS1B. (C) mRNA expression of PD-L1 was measured by qRT-PCR. (D) Protein levels of LC3B were measured by Western blotting. (E) Protein levels of p-STAT3 and PD-L1 were measured by western blotting.

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