产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3240, RRID:AB_2835620.
展开/折叠
mitochondrial; Mt SSB; Mt-SSB; MtSSB; PWP1 interacting protein 17; PWP1-interacting protein 17; SENSOR OF SINGLE-STRANDED DNA COMPLEX; Single stranded DNA binding protein 1; Single stranded DNA binding protein; Single stranded DNA binding protein mitochondrial; Single-stranded DNA-binding protein; SOSS B1; SOSS COMPLEX, SUBUNIT B1; SSBP 1; SSBP; SSBP_HUMAN; SSBP1;
抗原和靶标
- Q04837 SSBP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFRRPVLQVLRQFVRHESETTTSLVLERSLNRVHLLGRVGQDPVLRQVEGKNPVTIFSLATNEMWRSGDSEVYQLGDVSQKTTWHRISVFRPGLRDVAYQYVKKGSRIYLEGKIDYGEYMDKNNVRRQATTIIADNIIFLSDQTKEKE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q04837 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K51 | Acetylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S70 | Phosphorylation | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| Y99 | Phosphorylation | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| K103 | Acetylation | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| K113 | Acetylation | Uniprot | |
| Y119 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| T130 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot |
研究背景
Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays crucial roles during mtDNA replication that stimulate activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.
Mitochondrion. Mitochondrion matrix>Mitochondrion nucleoid.
Homotetramer. Interacts with MPG/AAG, through inhibition of its glycosylase activity it potentially prevents formation of DNA breaks in ssDNA, ensuring that base removal primarily occurs in dsDNA. Interacts with POLDIP2. Interacts with PRIMPOL.
研究领域
· Genetic Information Processing > Replication and repair > DNA replication.
· Genetic Information Processing > Replication and repair > Mismatch repair.
· Genetic Information Processing > Replication and repair > Homologous recombination.
限制条款
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