DYR1A Antibody - #DF3270

Fig. 4 Effect of DYRK1A on HG-induced inflammatory and diabetes wound healing. a Venn diagram showing the number of potential target genes for miR-221-3p in three major miRNA target gene databases. b Venn diagram showing the number of the genes overlapping across the four indicated groups. c Sequence alignment of the wild-type (WT) 3′-untranslated region (3′-UTR) of DYRK1A containing putative miR-221-3p-binding sites and the miR-221-3p sequence. Mutated (mut) 3ʹ-UTR of DYRK1A without the putative miR-221-3p-binding site. d Relative luciferase activities of plasmids carrying WT or mutant DYRK1A 3ʹ-UTR in HEK293T cells co-transfected with miRNA mimic negative control (NC) or miR-221-3p mimic (n = 3) were determined using the Luciferase Dual Reporter gene assay detection system. e Representative image and summary data showing protein expression levels of DYRK1A. Protein band intensities were normalized to β-Actin (n = 3). Summary data showing relative mRNA levels of IL-1β (f), IL-6 (g), IL-8 (h) and TNF-α (i). ACTB was used as the internal reference (n = 3). Representative photomicrographs (j) and summary data (k) showing changes in the area of excisional wounds on days 0, 2, 4, 8, and 10 in diabetic mice injected with either negative control (DM-Ctrl), DYRK1A siRNA (DM-si) or both DYRK1A siRNA and miR-221-3p agomir (DM-si+mi). The smallest scale division of the ruler is 1 mm in (j). Data are presented as mean ± SEM, n = 5–7. *P