产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3276, RRID:AB_2835644.
展开/折叠
E3 ubiquitin/ISG15 ligase TRIM25; EFP; Estrogen responsive finger protein; Estrogen-responsive finger protein; RING finger protein 147; RNF 147; RNF147; TRI25; TRI25_HUMAN; TRIM 25; Trim25; Tripartite motif containing 25; Tripartite motif containing protein 25; Tripartite motif protein TRIM25; Tripartite motif-containing protein 25; Tripartite motifcontaining25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Z147; Zinc finger protein 147 (estrogen responsive finger protein); Zinc finger protein 147; Zinc finger protein-147; ZNF 147; ZNF147;
抗原和靶标
- Q14258 TRI25_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAELCPLAEELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCRAVYQARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14258 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S46 | Phosphorylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| Y57 | Phosphorylation | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| Y245 | Phosphorylation | Uniprot | |
| T246 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K273 | Acetylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| Y278 | Phosphorylation | Uniprot | |
| K283 | Methylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K284 | Methylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| T290 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| K320 | Acetylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| Y323 | Phosphorylation | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| K402 | Acetylation | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| K416 | Methylation | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| K425 | Methylation | Uniprot | |
| K425 | Ubiquitination | Uniprot | |
| T427 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| T434 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| K437 | Ubiquitination | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| Y455 | Phosphorylation | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| K509 | Ubiquitination | Uniprot | |
| K567 | Acetylation | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K573 | Ubiquitination | Uniprot |
研究背景
Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3.
Auto-ISGylated.
Cytoplasm. Cytoplasm>Stress granule.
Expressed in breast tumors (at protein level). Ubiquitous.
Interacts (via SPRY domain) with DDX58 (via CARD domain). Interacts with ZFHX3. Interacts with NLRP12; this interaction reduces the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked DDX58 activation.
(Microbial infection) Interacts (via coiled coil) with influenza A virus NS1 protein; this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated DDX58 CARD ubiquitination, thereby suppressing DDX58 signal transduction.
The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
研究领域
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.