| 产品: | GCKR 抗体 |
| 货号: | DF3277 |
| 描述: | Rabbit polyclonal antibody to GCKR |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Rabbit, Dog |
| 分子量: | 68 KD; 69kD(Calculated). |
| 蛋白号: | Q14397 |
| RRID: | AB_2835645 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3277, RRID:AB_2835645.
展开/折叠
FGQTL5; GCK; GCKR; GCKR_HUMAN; GKRP; GLRE; glucokinase (hexokinase 4) regulator; Glucokinase; Glucokinase regulator; Glucokinase regulatory protein; Hexokinase 4 regulator; Hexokinase type IV; Hexokinase-4; Hexokinase-D; HK IV; HK4; OTTHUMP00000158533;
抗原和靶标
Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
- Q14397 GCKR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIVRLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASREGTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAVFLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFERAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECIHTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSLTEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLKKLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKILQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSDDIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEAVRSALAGPGQKRTADPLEILEPDVQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14397 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K164 | Acetylation | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot |
研究背景
Regulates glucokinase (GCK) by forming an inactive complex with this enzyme. Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.
Cytoplasm. Nucleus. Mitochondrion.
Note: Under low glucose concentrations, GCKR associates with GCK and the inactive complex is recruited to the hepatocyte nucleus.
Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
Interacts (fructose 6-phosphate bound form) with GCK.
Fructose 1-phosphate and fructose 6-phosphate compete for the same binding site.
Belongs to the GCKR family.
限制条款
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