| 产品: | DOK3 抗体 |
| 货号: | DF3331 |
| 描述: | Rabbit polyclonal antibody to DOK3 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Rabbit, Dog, Chicken |
| 分子量: | 58 KD; 53kD(Calculated). |
| 蛋白号: | Q7L591 |
| RRID: | AB_2835698 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3331, RRID:AB_2835698.
展开/折叠
Docking protein 3; DOK 3; DOK Like Protein; Dok3; DOK3_HUMAN; DOKL Pending; Downstream of tyrosine kinase 3; p62 DOK Like Protein;
抗原和靶标
- Q7L591 DOK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTRGARLRSDARAQLNQLSLDGGTGSGQKGKCEEFPSSLSSVSPGLEAAALLLAVTMDPLETPIKDGILYQQHVKFGKKCWRKVWALLYAGGPSGVARLESWEVRDGGLGAAGDRSAGPGRRGERRVIRLADCVSVLPADGESCPRDTGAFLLTTTERSHLLAAQHRQAWMGPICQLAFPGTGEASSGSTDAQSPKRGLVPMEENSIYSSWQEVGEFPVVVQRTEAATRCQLKGPALLVLGPDAIQLREAKGTQALYSWPYHFLRKFGSDKGVFSFEAGRRCHSGEGLFAFSTPCAPDLCRAVAGAIARQRERLPELTRPQPCPLPRATSLPSLDTPGELREMPPGPEPPTSRKMHLAEPGPQSLPLLLGPEPNDLASGLYASVCKRASGPPGNEHLYENLCVLEASPTLHGGEPEPHEGPGSRSPTTSPIYHNGQDLSWPGPANDSTLEAQYRRLLELDQVEGTGRPDPQAGFKAKLVTLLSRERRKGPAPCDRP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q7L591 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| Y89 | Phosphorylation | Uniprot | |
| R122 | Methylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| Y381 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| Y398 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| Y432 | Phosphorylation | Uniprot | |
| Y453 | Phosphorylation | Uniprot | |
| K477 | Acetylation | Uniprot | |
| T480 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot |
研究背景
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function (By similarity).
Constitutively tyrosine-phosphorylated.
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase (By similarity).
Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Expressed in spleen.
On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL1 through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP (By similarity).
PTB domain mediates receptor interaction.
Belongs to the DOK family. Type A subfamily.
限制条款
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