产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3427, RRID:AB_2835727.
展开/折叠
DKFZp434J2031; FLJ10181; ILKAP; ILKAP_HUMAN; ILKAP2; ILKAP3; Integrin linked kinase associated phosphatase; Integrin linked kinase associated serine/threonine phosphatase 2C; Integrin linked kinase associated serine/threonine phosphatase; Integrin-linked kinase-associated serine/threonine phosphatase 2C; MGC4846; PP2C DELTA; Protein phosphatase 2c delta isozyme;
抗原和靶标
Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.
- Q9H0C8 ILKAP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSSLITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRDGRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKIQTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H0C8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| T81 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K86 | Acetylation | Uniprot | |
| K95 | Acetylation | Uniprot | |
| K161 | Acetylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| C190 | S-Nitrosylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K204 | Methylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K210 | Acetylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| Y265 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| R319 | Methylation | Uniprot | |
| R369 | Methylation | Uniprot | |
| R377 | Methylation | Uniprot |
研究背景
Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.
Cytoplasm.
Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.
Interacts with ILK. Specific association with ILK is independent of the catalytic activity of either partner.
Belongs to the PP2C family.
限制条款
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