产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0214, RRID:AB_2833284.
展开/折叠
C8orf57; DKFZp761D112; Matrix metalloproteinase 16; Matrix metalloproteinase-16; Membrane type 3 matrix metalloproteinase; Membrane type matrix metalloproteinase 3; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP 16; MMP-16; MMP-X2; MMP16; MMP16_HUMAN; MT-MMP 3; MT-MMP2; MT-MMP3; MT3-MMP; MT3MMP; MTMMP2; MTMMP3; Putative transmembrane protein C8orf57;
抗原和靶标
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
- P51512 MMP16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MILLTFSTGRRLDFVHHSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQSALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFHIRRKRYALTGQKWQHKHITYSIKNVTPKVGDPETRKAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSIPPADPRKNDRPKPPRPPTGRPSYPGAKPNICDGNFNTLAILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQPGYPHDLITLGSGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITVWKGIPESPQGAFVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRVKEGHSPPDDVDIVIKLDNTASTVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P51512 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T5 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| T92 | Phosphorylation | Uniprot | |
| Y134 | Phosphorylation | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| T382 | Phosphorylation | Uniprot | |
| T478 | Phosphorylation | Uniprot | |
| Y521 | Phosphorylation | Uniprot |
研究背景
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
The precursor is cleaved by a furin endopeptidase.
Cell membrane>Single-pass type I membrane protein>Extracellular side.
Note: Localized at the cell surface of melanoma cells.
Secreted>Extracellular space>Extracellular matrix. Cell surface.
Note: Localized at the cell surface of melanoma cells.
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Belongs to the peptidase M10A family.
研究领域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
限制条款
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