产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3430, RRID:AB_2835730.
展开/折叠
5NT3A_HUMAN; cN III; cN-III; cNIII; Cytosolic 5' nucleotidase 3; Cytosolic 5' nucleotidase III; Cytosolic 5''-nucleotidase 3; Cytosolic 5''-nucleotidase 3A; Cytosolic 5''-nucleotidase III; Nt5c3a; p36; P5''N-1; P5'N 1; P5'N1; P5N 1; P5N-1; P5N1; PN I; PN-I; PNI; PSN1; Pyrimidine 5' nucleotidase 1; Pyrimidine 5''-nucleotidase 1; UMPH; UMPH1; Uridine 5' monophosphate hydrolase 1; Uridine 5''-monophosphate hydrolase 1;
抗原和靶标
Isoforms 1, 3 and 4 are expressed in reticulocytes. Isoform 4 is hardly detectable in bone marrow and fetal liver.
- Q9H0P0 5NT3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRAPSMDRAAVARVGAVASASVCALVAGVVLAQYIFTLKRKTGRKTKIIEMMPEFQKSSVRIKNPTRVEEIICGLIKGGAAKLQIITDFDMTLSRFSYKGKRCPTCHNIIDNCKLVTDECRKKLLQLKEKYYAIEVDPVLTVEEKYPYMVEWYTKSHGLLVQQALPKAKLKEIVAESDVMLKEGYENFFDKLQQHSIPVFIFSAGIGDVLEEVIRQAGVYHPNVKVVSNFMDFDETGVLKGFKGELIHVFNKHDGALRNTEYFNQLKDNSNIILLGDSQGDLRMADGVANVEHILKIGYLNDRVDELLEKYMDSYDIVLVQDESLEVANSILQKIL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H0P0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K47 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| R303 | Methylation | Uniprot | |
| K334 | Ubiquitination | Uniprot |
研究背景
Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP). CMP seems to be the preferred substrate.
Cytoplasm.
Endoplasmic reticulum.
Isoforms 1, 3 and 4 are expressed in reticulocytes. Isoform 4 is hardly detectable in bone marrow and fetal liver.
Monomer.
Belongs to the pyrimidine 5'-nucleotidase family.
研究领域
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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