CDH11 Antibody - #DF3523

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产品: CDH11 抗体
货号: DF3523
描述: Rabbit polyclonal antibody to CDH11
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 80,100kD; 88kD(Calculated).
蛋白号: P55287
RRID: AB_2835743

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
克隆:
Polyclonal
特异性:
CDH11 Antibody detects endogenous levels of total CDH11.
RRID:
AB_2835743
引用格式: Affinity Biosciences Cat# DF3523, RRID:AB_2835743.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CAD11; CAD11_HUMAN; Cadherin 11; Cadherin 11 type 2; Cadherin 11 type 2 OB cadherin (osteoblast); Cadherin-11; Cdh11; CDHOB; OB; OB-cadherin; OBcadherin; OSF-4; OSF4; Osteoblast cadherin;

抗原和靶标

免疫原:
Uniprot:

P55287(CAD11_HUMAN) >>Visit HPA database.

基因/基因ID:
CDH11(1009)
表达:
P55287 CAD11_HUMAN:

Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts.

序列:
MKENYCLQAALVCLGMLCHSHAFAPERRGHLRPSFHGHHEKGKEGQVLQRSKRGWVWNQFFVIEEYTGPDPVLVGRLHSDIDSGDGNIKYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQYTLMAQAVDRDTNRPLEPPSEFIVKVQDINDNPPEFLHETYHANVPERSNVGTSVIQVTASDADDPTYGNSAKLVYSILEGQPYFSVEAQTGIIRTALPNMDREAKEEYHVVIQAKDMGGHMGGLSGTTKVTITLTDVNDNPPKFPQSVYQMSVSEAAVPGEEVGRVKAKDPDIGENGLVTYNIVDGDGMESFEITTDYETQEGVIKLKKPVDFETKRAYSLKVEAANVHIDPKFISNGPFKDTVTVKISVEDADEPPMFLAPSYIHEVQENAAAGTVVGRVHAKDPDAANSPIRYSIDRHTDLDRFFTINPEDGFIKTTKPLDREETAWLNITVFAAEIHNRHQEAKVPVAIRVLDVNDNAPKFAAPYEGFICESDQTKPLSNQPIVTISADDKDDTANGPRFIFSLPPEIIHNPNFTVRDNRDNTAGVYARRGGFSRQKQDLYLLPIVISDGGIPPMSSTNTLTIKVCGCDVNGALLSCNAEAYILNAGLSTGALIAILACIVILLVIVVLFVTLRRQKKEPLIVFEEEDVRENIITYDDEGGGEEDTEAFDIATLQNPDGINGFIPRKDIKPEYQYMPRPGLRPAPNSVDVDDFINTRIQEADNDPTAPPYDSIQIYGYEGRGSVAGSLSSLESATTDSDLDYDYLQNWGPRFKKLADLYGSKDTFDDDS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Zebrafish
78
Xenopus
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P55287 作为底物

Site PTM Type Enzyme
K43 Acetylation
S93 Phosphorylation
T99 Phosphorylation
T219 Phosphorylation
K365 Ubiquitination
Y700 Phosphorylation
Y702 Phosphorylation
S714 Phosphorylation
T723 Phosphorylation
S788 Phosphorylation
T791 Phosphorylation

研究背景

功能:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

细胞定位:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts.

亚基结构:

Interacts with PCDH8.

蛋白家族:

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

文献引用

1). MiRNA-451a inhibits airway remodeling by targeting Cadherin 11 in an allergic asthma model of neonatal mice. International Immunopharmacology, 2020 (PubMed: 32234673) [IF=5.6]

Application: IF/ICC    Species: Mouse    Sample: lung tissues

Fig. 1. Opposite expression of miRNA-451a and CDH11 in the lung of asthma mouse pups. (A) New born mice were intraperitoneally injected with 10 μg ovalbumin (OVA) from postnatal day 5 (P5) to P10, and followed by 10-min daily challenge with 3% aerosolized OVA from P18 to P20. Twenty-four hours post the last OVA challenge (P21), the bronchoalveolar lavage fluid (BALF) was collected, and then the mice were sacrificed to collect their lung tissues. CD 4+ T cells were isolated from the control and asthmatic BALF samples. The expression levels of miRNA-451a in (B) CD 4+ T cells and (C) lung tissues were determined with real-time qPCR. The mRNA and protein levels of CDH11 were determined with (D) real-time qPCR, (E) western blot, and (F) immunofluorescent staining (bars, 50 μm). Data were expressed as mean ± S.D., and p < 0.05 was regarded statistically significant. *, **, *** indicated a p < 0.05, 0.01, 0.001.

Application: WB    Species: Mouse    Sample: lung tissues

Fig. 1. Opposite expression of miRNA-451a and CDH11 in the lung of asthma mouse pups. (A) New born mice were intraperitoneally injected with 10 μg ovalbumin (OVA) from postnatal day 5 (P5) to P10, and followed by 10-min daily challenge with 3% aerosolized OVA from P18 to P20. Twenty-four hours post the last OVA challenge (P21), the bronchoalveolar lavage fluid (BALF) was collected, and then the mice were sacrificed to collect their lung tissues. CD 4+ T cells were isolated from the control and asthmatic BALF samples. The expression levels of miRNA-451a in (B) CD 4+ T cells and (C) lung tissues were determined with real-time qPCR. The mRNA and protein levels of CDH11 were determined with (D) real-time qPCR, (E) western blot, and (F) immunofluorescent staining (bars, 50 μm). Data were expressed as mean ± S.D., and p < 0.05 was regarded statistically significant. *, **, *** indicated a p < 0.05, 0.01, 0.001.
2). BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo. MOLECULAR CARCINOGENESIS, 2017 (PubMed: 27770580) [IF=4.6]

Application: WB    Species: human    Sample: HepG2

Figure 6. Effect of BCL2L10 on its downstream gene expression profiles of human cancer pathway in HepG2 cells. (A) By human cancer pathway PCR array, ectopic expression of BCL2L10 up- or down-regulated several genes related to tumor proliferation, apoptosis, metastasis and angiogenesis. (B) Western blot was performed to confirm the downstream gene expression regulated by BCL2L10 in HepG2 cells. GAPDH was used as an internal control. (C) Schematic diagram of the molecular events for BCL2L10 function as a tumor suppressor through regulating cell cycle, proliferation, apoptosis metastasis and angiogenesis effectors.

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