产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3448, RRID:AB_2835814.
展开/折叠
2010002H18Rik; C79125; EC 6.1.1.5; FLJ10326; Iars2; IleRS; isoleucine tRNA ligase 2, mitochondrial; isoleucine tRNA ligase, mitochondrial; isoleucine tRNA synthetase 2, mitochondrial; Isoleucine--tRNA ligase; isoleucine-tRNA synthetase 2; isoleucyl tRNA synthetase 2, mitochondrial; Isoleucyl-tRNA synthetase; MGC63429; mitochondrial; mitochondrial isoleucine tRNA synthetase; rCG_20084; RGD1311857; SYIM_HUMAN;
抗原和靶标
- Q9NSE4 SYIM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRWGLRPRGPGAAALATARSLWGTPRLPCSPGWQGATKRLLVRSVSGASNHQPNSNSGRYRDTVLLPQTSFPMKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFHMMNGSKIHFVPGWDCHGLPIEIKVLSELGREAQNLSAMEIRKKARSFAKAAIEKQKSAFIRWGIMADWNNCYYTFDGKYEAKQLRTFYQMYDKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSPKLASLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAADKVASVASTLETTFETISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVHTAPAHGMEDYGVASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKTAAKELLKKVKFIPGSALNGMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIVKLVEQHGSDIWWTLPPEQLLPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEGKDQLGGWFQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPDVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NSE4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S44 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K189 | Acetylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K222 | Acetylation | Uniprot | |
| R225 | Methylation | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| K233 | Acetylation | Uniprot | |
| Y237 | Phosphorylation | Uniprot | |
| K241 | Acetylation | Uniprot | |
| K241 | Methylation | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| Y260 | Phosphorylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| K455 | Sumoylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K479 | Acetylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| K500 | Acetylation | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| Y543 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| Y621 | Phosphorylation | Uniprot | |
| T657 | Phosphorylation | Uniprot | |
| K687 | Ubiquitination | Uniprot | |
| K725 | Acetylation | Uniprot | |
| K775 | Acetylation | Uniprot | |
| K781 | Acetylation | Uniprot | |
| Y798 | Phosphorylation | Uniprot | |
| K803 | Acetylation | Uniprot | |
| K872 | Ubiquitination | Uniprot | |
| S881 | Phosphorylation | Uniprot | |
| S888 | Phosphorylation | Uniprot | |
| K896 | Ubiquitination | Uniprot | |
| T950 | Phosphorylation | Uniprot | |
| T982 | Phosphorylation | Uniprot | |
| C1005 | S-Nitrosylation | Uniprot |
研究背景
Mitochondrion matrix.
Belongs to the class-I aminoacyl-tRNA synthetase family.
研究领域
· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.
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