| 产品: | MBD3 抗体 |
| 货号: | AF0353 |
| 描述: | Rabbit polyclonal antibody to MBD3 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Chicken |
| 分子量: | 33kDa; 33kD(Calculated). |
| 蛋白号: | O95983 |
| RRID: | AB_2833519 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0353, RRID:AB_2833519.
展开/折叠
AI181826; AU019209; MBD 3; Mbd3; MBD3: methyl CpG binding domain protein 3; MBD3_HUMAN; Methyl CpG binding domain protein 3; Methyl CpG binding protein MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3;
抗原和靶标
A synthesized peptide derived from human MBD3, corresponding to a region within C-terminal amino acids.
- O95983 MBD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERKRWECPALPQGWEREEVPRRSGLSAGHRDVFYYSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKNPGVWLNTTQPLCKAFMVTDEDIRKQEELVQQVRKRLEEALMADMLAHVEELARDGEAPLDKACAEDDDEEDEEEEEEEPDPDPEMEHV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95983 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S24 | Phosphorylation | O14965 (AURKA) | Uniprot |
| Y35 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| T66 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | O14965 (AURKA) | Uniprot |
| S86 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| K109 | Acetylation | Uniprot | |
| K109 | Sumoylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K141 | Acetylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| T221 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot |
研究背景
Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides. Recruits histone deacetylases and DNA methyltransferases.
Nucleus. Chromosome.
Note: Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes.
Heterodimer with MBD2. Part of the NuRD and the MeCP1 complex. Interacts with BCL6, HDAC1, MTA2, DNMT1, p66-alpha and p66-beta.
限制条款
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