产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3476, RRID:AB_2835839.
展开/折叠
Transcription factor ZBP-89; Transcription factor ZBP89; ZBP89; Zinc finger DNA binding protein 89; Zinc finger DNA-binding protein 89; Zinc finger protein 148; ZN148_HUMAN; ZNF 148; ZNF148;
抗原和靶标
- Q9UQR1 ZN148_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNIDDKLEGLFLKCGGIDEMQSSRTMVVMGGVSGQSTVSGELQDSVLQDRSMPHQEILAADEVLQESEMRQQDMISHDELMVHEETVKNDEEQMETHERLPQGLQYALNVPISVKQEITFTDVSEQLMRDKKQIREPVDLQKKKKRKQRSPAKILTINEDGSLGLKTPKSHVCEHCNAAFRTNYHLQRHVFIHTGEKPFQCSQCDMRFIQKYLLQRHEKIHTGEKPFRCDECGMRFIQKYHMERHKRTHSGEKPYQCEYCLQYFSRTDRVLKHKRMCHENHDKKLNRCAIKGGLLTSEEDSGFSTSPKDNSLPKKKRQKTEKKSSGMDKESALDKSDLKKDKNDYLPLYSSSTKVKDEYMVAEYAVEMPHSSVGGSHLEDASGEIHPPKLVLKKINSKRSLKQPLEQNQTISPLSTYEESKVSKYAFELVDKQALLDSEGNADIDQVDNLQEGPSKPVHSSTNYDDAMQFLKKKRYLQAASNNSREYALNVGTIASQPSVTQAAVASVIDESTTASILESQALNVEIKSNHDKNVIPDEVLQTLLDHYSHKANGQHEISFSVADTEVTSSISINSSEVPEVTPSENVGSSSQASSSDKANMLQEYSKFLQQALDRTSQNDAYLNSPSLNFVTDNQTLPNQPAFSSIDKQVYATMPINSFRSGMNSPLRTTPDKSHFGLIVGDSQHSFPFSGDETNHASATSTQDFLDQVTSQKKAEAQPVHQAYQMSSFEQPFRAPYHGSRAGIATQFSTANGQVNLRGPGTSAEFSEFPLVNVNDNRAGMTSSPDATTGQTFG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UQR1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T25 | Phosphorylation | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| K115 | Sumoylation | Uniprot | |
| K132 | Sumoylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| T167 | Phosphorylation | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| S202 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T222 | Phosphorylation | Uniprot | |
| K239 | Sumoylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| Y259 | Phosphorylation | Uniprot | |
| K291 | Methylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| T296 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| Y345 | Phosphorylation | Uniprot | |
| Y349 | Phosphorylation | Uniprot | |
| K356 | Sumoylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| K424 | Sumoylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| Y425 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| K456 | Acetylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| K607 | Acetylation | Uniprot | |
| K607 | Ubiquitination | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| T669 | Phosphorylation | Uniprot | |
| T670 | Phosphorylation | Uniprot | |
| R734 | Methylation | Uniprot | |
| S740 | Phosphorylation | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| S784 | Phosphorylation | Uniprot |
研究背景
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes (By similarity).
Nucleus.
Interacts with HNRNPDL. Interacts with the 5FMC complex; the interaction requires association with CHTOP. Interacts with CAVIN1 (By similarity).
Belongs to the krueppel C2H2-type zinc-finger protein family.
限制条款
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