产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3484, RRID:AB_2835847.
展开/折叠
AOMS3; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B; Dual specificity tyrosine phosphorylation regulated kinase 1B; Dual specificity tyrosine-phosphorylation-regulated kinase 1B; DYR 1B; DYR1A; DYR1B; DYR1B_HUMAN; DYRK 1B; Dyrk1b; Minibrain related kinase; Minibrain-related kinase; MIRK; Mirk protein kinase;
抗原和靶标
Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.
- Q9Y463 DYR1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRRAQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERLPGGGWTLRRTKELRKDYQGPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPSPTSPPPPELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPHLGLRGVPQSTAASS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y463 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S49 | Phosphorylation | Uniprot | |
| K57 | Acetylation | Uniprot | |
| Y63 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| Y271 | Phosphorylation | Q9Y463 (DYRK1B) | Uniprot |
| Y273 | Phosphorylation | Q9Y463 (DYRK1B) | Uniprot |
| S276 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Q9Y463 (DYRK1B) , P28482 (MAPK1) | Uniprot |
| Y485 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot |
翻译修饰 - Q9Y463 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O75533 (SF3B1) | T434 | Uniprot |
| P20823 (HNF1A) | S249 | Uniprot |
| P24385 (CCND1) | T286 | Uniprot |
| P24385 (CCND1) | T288 | Uniprot |
| P38936 (CDKN1A) | S153 | Uniprot |
| P46527 (CDKN1B) | S10 | Uniprot |
| Q02363 (ID2) | T27 | Uniprot |
| Q99801 (NKX3-1) | S185 | Uniprot |
| Q9UQL6 (HDAC5) | S279 | Uniprot |
| Q9Y463 (DYRK1B) | Y271 | Uniprot |
| Q9Y463 (DYRK1B) | Y273 | Uniprot |
| Q9Y463 (DYRK1B) | S421 | Uniprot |
研究背景
Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. Inhibits the SHH and WNT1 pathways, thereby enhancing adipogenesis. In addition, promotes expression of the gluconeogenic enzyme glucose-6-phosphatase (G6PC).
Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization.
Nucleus.
Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.
Dimer. Interacts with DCOHM, MAP2K3/MKK3, RANBP9 and TCF1/HNF1A. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5. Interacts with DCAF7.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
限制条款
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