产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3491, RRID:AB_2835853.
展开/折叠
14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 protein epsilon; 14-3-3E; 1433E_HUMAN; Epididymis luminal protein 2; FLJ45465; FLJ53559; HEL2; KCIP 1; KCIP1; MDCR; MDS; Mitochondrial import stimulation factor L subunit; Protein kinase C inhibitor protein1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide; YWHAE;
抗原和靶标
- P62258 1433E_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P62258 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| R4 | Methylation | Uniprot | |
| K12 | Acetylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| K50 | Acetylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| C97 | S-Nitrosylation | Uniprot | |
| C98 | S-Nitrosylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| Y122 | Phosphorylation | Uniprot | |
| K123 | Acetylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| R130 | Methylation | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| K142 | Acetylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K153 | Methylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| T208 | Phosphorylation | Q9NYY3 (PLK2) , Q9H4B4 (PLK3) | Uniprot |
| S210 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| Y214 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| K244 | Ubiquitination | Uniprot |
研究背景
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.
Nucleus. Cytoplasm. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Homodimer. Heterodimerizes with YWHAZ. Interacts with PKA-phosphorylated AANAT. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with ARHGEF28 (By similarity). Interacts with BEX3 (By similarity). Weakly interacts with CDKN1B. Interacts with the 'Thr-369' phosphorylated form of DAPK2. Interacts with DENND1A. Interacts with GAB2. Interacts with phosphorylated GRB10. Interacts with KSR1. Interacts with NDEL1 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with the phosphorylated (by AKT1) form of SRPK2. Interacts with TIAM2. Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (By similarity). Interacts with ZFP36 (via phosphorylated form) (By similarity). Interacts with SLITRK1. Interacts with HSF1 (via phosphorylated form); this interaction promotes HSF1 sequestration in the cytoplasm in a ERK-dependent manner. Interacts with RIPOR2 isoform 2. Interacts with KLHL22; required for the nuclear localization of KLHL22 upon amino acid starvation. Interacts with CRTC1. Interacts with CRTC2 (probably when phosphorylated at 'Ser-171'). Interacts with CRTC3 (probably when phosphorylated at 'Ser-162' and/or 'Ser-273'). Interacts with ATP2B1; this interaction inhibits calcium-transporting ATPase activity.
(Microbial infection) Interacts with HCV core protein.
Belongs to the 14-3-3 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
限制条款
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