| 产品: | 14-3-3 eta 抗体 |
| 货号: | DF3492 |
| 描述: | Rabbit polyclonal antibody to 14-3-3 eta |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 42 KD; 28kD(Calculated). |
| 蛋白号: | Q04917 |
| RRID: | AB_2835854 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3492, RRID:AB_2835854.
展开/折叠
14 3 3 protein eta; 14-3-3 eta; 14-3-3 protein eta; 1433F_HUMAN; Brain protein 14-3-3, eta isoform; HGNC:12853; Protein AS1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide; YWHA 1; YWHA1; Ywhah;
抗原和靶标
Expressed mainly in the brain and present in other tissues albeit at lower levels.
- Q04917 1433F_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDREQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q04917 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y20 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| R42 | Methylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| K50 | Acetylation | Uniprot | |
| K50 | Methylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| S59 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| S65 | Phosphorylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K120 | Acetylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| Y123 | Phosphorylation | Uniprot | |
| K125 | Acetylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| Y130 | Phosphorylation | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| K142 | Acetylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| Y154 | Phosphorylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| Y216 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| T220 | Phosphorylation | Uniprot |
研究背景
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Expressed mainly in the brain and present in other tissues albeit at lower levels.
Homodimer (By similarity). Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with ARHGEF28 and CDK16 (By similarity). Weakly interacts with CDKN1B. Interacts with GAB2. Interacts with KCNK18 in a phosphorylation-dependent manner. Interacts with SAMSN1 (By similarity). Interacts with the 'Ser-241' phosphorylated form of PDPK1. Interacts with the 'Thr-369' phosphorylated form of DAPK2. Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with SLITRK1.
Belongs to the 14-3-3 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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