产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3493, RRID:AB_2835855.
展开/折叠
14 3 3 gamma; 14 3 3 protein gamma; 14 3 3 protein gamma subtype; 14 3 3gamma; 14-3-3 protein gamma; 1433G_HUMAN; 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide; KCIP 1; KCIP-1; KCIP1; N-terminally processed; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide; Ywhag;
抗原和靶标
- P61981 1433G_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVDREQLVQKARLAEQAERYDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTHPIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDDDGGEGNN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61981 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| V2 | Acetylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| Y20 | Phosphorylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| T31 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| K50 | Acetylation | Uniprot | |
| K50 | Methylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S59 | Phosphorylation | Q9NRM7 (LATS2) | Uniprot |
| S65 | Phosphorylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| Y117 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K120 | Acetylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K125 | Acetylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| Y130 | Phosphorylation | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| Y133 | Phosphorylation | Uniprot | |
| T139 | Phosphorylation | Uniprot | |
| K142 | Acetylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K152 | Acetylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K162 | Acetylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| Y216 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| T220 | Phosphorylation | Uniprot | |
| T234 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot |
研究背景
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Phosphorylated by various PKC isozymes.
Cytoplasm.
Highly expressed in brain, skeletal muscle, and heart.
Homodimer. Interacts with SAMSN1 (By similarity). Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT and SIRT2.Interacts with the 'Thr-369' phosphorylated form of DAPK2. Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with SLITRK1. Interacts with LRRK2; this interaction is dependent on LRRK2 phosphorylation. Interacts with MARK2 and MARK3.
Belongs to the 14-3-3 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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