5HT1F Receptor Antibody - #DF3499

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产品: 5HT1F Receptor 抗体
货号: DF3499
描述: Rabbit polyclonal antibody to 5HT1F Receptor
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 38 KD; 42kD(Calculated).
蛋白号: P30939
RRID: AB_2835861

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(82%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
5HT1F Receptor Antibody detects endogenous levels of total 5HT1F Receptor.
RRID:
AB_2835861
引用格式: Affinity Biosciences Cat# DF3499, RRID:AB_2835861.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5 HT 1F; 5 HT1F; 5 hydroxytryptamine (serotonin) receptor 1F; 5 hydroxytryptamine 1F receptor; 5 hydroxytryptamine receptor 1E beta; 5 hydroxytryptamine receptor 1F; 5 hydroxytryptamine serotonin receptor 1E beta; 5 hydroxytryptamine serotonin receptor 1F; 5HT 1F; 5HT1F; HGNC5292; HTR 1F; HTR1EB; HTR1EL; HTR1F; MR 77; MR77; Serotonin receptor 1E beta; Serotonin receptor gene;

抗原和靶标

免疫原:
Uniprot:

P30939(5HT1F_HUMAN) >>Visit HPA database.

基因/基因ID:
HTR1F(3355)
序列:
MDFLNSSDQNLTSEELLNRMPSKILVSLTLSGLALMTTTINSLVIAAIIVTRKLHHPANYLICSLAVTDFLVAVLVMPFSIVYIVRESWIMGQVVCDIWLSVDITCCTCSILHLSAIALDRYRAITDAVEYARKRTPKHAGIMITIVWIISVFISMPPLFWRHQGTSRDDECIIKHDHIVSTIYSTFGAFYIPLALILILYYKIYRAAKTLYHKRQASRIAKEEVNGQVLLESGEKSTKSVSTSYVLEKSLSDPSTDFDKIHSTVRSLRSEFKHEKSWRRQKISGTRERKAATTLGLILGAFVICWLPFFVKELVVNVCDKCKISEEMSNFLAWLGYLNSLINPLIYTIFNEDFKKAFQKLVRCRC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P30939 作为底物

Site PTM Type Enzyme
S27 Phosphorylation
T38 Phosphorylation
Y122 Phosphorylation
T126 Phosphorylation
Y131 Phosphorylation
Y184 Phosphorylation
Y201 Phosphorylation
Y202 Phosphorylation
Y205 Phosphorylation
S233 Phosphorylation

研究背景

功能:

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

文献引用

1). 5-HT1F Receptor Agonist Ameliorates Mechanical Allodynia in Neuropathic Pain via Induction of Mitochondrial Biogenesis and Suppression of Neuroinflammation. Frontiers in Pharmacology, 2022 (PubMed: 35308244) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 3 Expression of 5-HT1F receptor in the spinal cord of sham and neuropathic pain rats. (A) The protein level of 5-HT1F receptor was significantly decreased in SNI rats, beginning at day 3 after surgery and remain at low level until day 14 (** p < 0.01 compared with sham group, n = 6 in each group). (B) Histogram showing the mean intensity of 5-HT1F receptor immunofluorescent activity was significantly decreased in SNI rats, beginning at day 3 after surgery and remain at low level until day 14 (****p < 0.0001 compared with sham group, n = 6 in each group). (C–H) Representative photomicrographs showed that neuropathic pain induced the downregulation of 5-HT1F receptor in the spinal cord.

Application: IF/ICC    Species: Rat    Sample:

FIGURE 4 Double immunofluorescence of 5-HT1F receptor and NeuN, GFAP, and Iba1 in the spinal cord of sham and neuropathic pain rats. (A) Double immunofluorescence of 5-HT1F receptor and NeuN in the spinal cord of sham rats. (B) Double immunofluorescence of 5-HT1F receptor and Iba-1 in the spinal cord of sham rats. (C) Double immunofluorescence of 5-HT1F receptor and GFAP in the spinal cord of sham rats. (D) Double immunofluorescence of 5-HT1F receptor and NeuN in the spinal cord of SNI rats. (E) Double immunofluorescence of 5-HT1F receptor and Iba-1 in the spinal cord of SNI rats. (F) Double immunofluorescence of 5-HT1F receptor and GFAP in the spinal cord of SNI rats. (G–I) Neuropathic pain induced decreased colocalization of 5-HT1F receptor with neurons (NeuN), while the colocalization of 5-HT1F receptor with astrocytes (GFAP), and microglia (Iba-1) remained sparse in the spinal cord (*p < 0.05 compared with sham group, n = 6 in each group).

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