5-HT-4 Antibody - #DF3503

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产品: 5-HT-4 抗体
货号: DF3503
描述: Rabbit polyclonal antibody to 5-HT-4
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Rabbit
分子量: 43 KD; 44kD(Calculated).
蛋白号: Q13639
RRID: AB_2835865

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(82%), Sheep(82%), Rabbit(91%)
克隆:
Polyclonal
特异性:
5-HT-4 Antibody detects endogenous levels of total 5-HT-4.
RRID:
AB_2835865
引用格式: Affinity Biosciences Cat# DF3503, RRID:AB_2835865.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5 HT 4; 5 HT4; 5 HT4R; 5 hydroxytryptamine (serotonin) receptor 4, G protein coupled; 5 hydroxytryptamine 4 receptor; 5 hydroxytryptamine receptor 4; 5 hydroxytryptamine serotonin receptor 4; 5 hydroxytryptamine4 receptor; 5-HT-4; 5-ht4; 5-HT4R; 5H4; 5HT 4; 5HT4; 5ht4 receptor; 5HT4R; Cardiac 5 HT4 receptor; HTR 4; HTR4; serotonin 4 receptor; Serotonin 5 HT4 receptor; serotonin 5-ht-4 receptor; serotonin 5-ht-4a; Serotonin receptor 4;

抗原和靶标

免疫原:
Uniprot:

Q13639(5HT4R_HUMAN) >>Visit HPA database.

基因/基因ID:
HTR4(3360)
表达:
Q13639 5HT4R_HUMAN:

Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle.

序列:
MDKLDANVSSEEGFGSVEKVVLLTFLSTVILMAILGNLLVMVAVCWDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAIELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLCCISLDRYYAICCQPLVYRNKMTPLRIALMLGGCWVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNSNSTYCVFMVNKPYAITCSVVAFYIPFLLMVLAYYRIYVTAKEHAHQIQMLQRAGASSESRPQSADQHSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTNIVDPFIDYTVPGQVWTAFLWLGYINSGLNPFLYAFLNKSFRRAFLIILCCDDERYRRPSILGQTVPCSTTTINGSTHVLRDAVECGGQWESQCHPPATSPLVAAQPSDT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
91
Bovine
82
Sheep
82
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13639 作为底物

Site PTM Type Enzyme
S354 Phosphorylation
T355 Phosphorylation

研究背景

功能:

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.

细胞定位:

Cell membrane>Multi-pass membrane protein. Endosome.
Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle.

亚基结构:

Isoform 5-HT4(A) interacts with MAGI2, MPP3, SLC9A3R1 and SNX27 isoforms 1 and 2. Isoform 5-HT4(E) interacts with PATJ, NOS1 and SEC23A. Isoform 5-HT4(A) forms a complex including SLC9A3R1 and EZR (By similarity). Interacts (via C-terminus 330-346 AA) with GRK5; this interaction is promoted by 5-HT (serotonin).

蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

研究领域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Serotonergic synapse.

文献引用

1). Bilirubin Induces Pain Desensitization in Cholestasis by Activating 5-Hydroxytryptamine 3A Receptor in Spinal Cord. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 33869168) [IF=5.5]

Application: WB    Species: rat    Sample: spinal cord

FIGURE 2 | Bilirubin increased 5-HT3A receptor expression and neuron activities in the spinal cord. (C) Intrathecal administration of bilirubin increased 5-HT3A receptor expression without changing other subtypes.

Application: WB    Species: Rat    Sample: spinal cords

FIGURE 2 Bilirubin increased 5-HT3A receptor expression and neuron activities in the spinal cord. (A) 5-HT3A receptor expression increased gradually in spinal cord enlargement of BDL rats, whereas expression of 5-HT3B, 5-HT3C, 5-HT3D, and 5-HT3E receptors did not change significantly. (B) The quantitative analysis of 5-HT3A receptor expression in BDL rats. (C) Intrathecal administration of bilirubin increased 5-HT3A receptor expression without changing other subtypes. (D) The quantitative analysis of 5-HT3A receptor expression after intrathecal administration of bilirubin in normal rats. (E) SDHNs cultured with bilirubin showed increased 5-HT3A receptor expression without changing other subtypes. (F) The quantitative analysis of 5-HT3A receptor expression in SDHNs. (G) Immunofluorescence showed that 5-HT3A receptor expression increased in laminas I and II in the spinal cords of BDL rats. The increased C-Fos protein (marker of neuron activity) expression indicated that neuron activities were also enhanced in the spinal cords of BDL rats. *P < 0.05.
2). Prucalopride might improve intestinal motility by promoting the regeneration of the enteric nervous system in diabetic rats. International Journal of Molecular Medicine, 2022 (PubMed: 35543167) [IF=5.4]

Application: IF/ICC    Species: Rat    Sample:

Figure 5 Immunofluorescence double staining images of Nestin and 5-HT4 in the Control, DM, DM + A and DM + B groups. DAPI-stained nuclei appeared blue. Nestin and 5-HT4 are indicated by red fluorescence and green fluorescence, respectively. A rat model of DM was established using an intraperitoneal injection of streptozotocin. The rats in the Control group were given an equal volume of citric acid solvent. Magnification, ×100. *P<0.05 vs. Control; #P<0.05 vs. DM. DM, diabetes mellitus model; DM + A, diabetic rats treated with 5 µg/kg prucalopride; DM + B, diabetic rats treated with 10 µg/kg prucalopride; 5-HT4, 5-hydroxytryptamine 4.
3). Qi Lang formula relieves constipation via targeting SCF/c-kit signaling pathway: An integrated study of network pharmacology and experimental validation. Heliyon, 2024 (PubMed: 38841509) [IF=4.0]
4). scRNA-Seq and Network Pharmacology Analysis Reveal SCF/C-kit as the Targeting Mechanism of Qi Lang Formula Relieving Constipation. , 2024

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