(1)
(2)
| 产品: | ARRDC3 抗体 |
| 货号: | DF3517 |
| 描述: | Rabbit polyclonal antibody to ARRDC3 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 46 KD; 46kD(Calculated). |
| 蛋白号: | Q96B67 |
| RRID: | AB_2835879 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3517, RRID:AB_2835879.
展开/折叠
ARRDC 3; Arrestin domain containing 3; Arrestin domain containing protein 3; KIAA1376; TBP-2-like inducible membrane protein; Thioredoxin binding protein 2 like inducible membrane; TLIMP;
抗原和靶标
Highly expressed in skeletal muscle, placenta, kidney, lung, liver, blood, adrenal gland, lymph node, mammary gland, thyroid, and trachea (PubMed:16269462, PubMed:21982743). Very low levels in colon, thymus, spleen, small intestine, bladder and bone marrow (PubMed:16269462). Strong expression in differentiated adipocytes compared to preadipocytes (PubMed:16269462). Detected in omental fat and subcutaneous fat tissue (PubMed:21982743).
- Q96B67 ARRD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVLGKVKSLTISFDCLNDSNVPVYSSGDTVSGRVNLEVTGEIRVKSLKIHARGHAKVRWTESRNAGSNTAYTQNYTEEVEYFNHKDILIGHERDDDNSEEGFHTIHSGRHEYAFSFELPQTPLATSFEGRHGSVRYWVKAELHRPWLLPVKLKKEFTVFEHIDINTPSLLSPQAGTKEKTLCCWFCTSGPISLSAKIERKGYTPGESIQIFAEIENCSSRMVVPKAAIYQTQAFYAKGKMKEVKQLVANLRGESLSSGKTETWNGKLLKIPPVSPSILDCSIIRVEYSLMVYVDIPGAMDLFLNLPLVIGTIPLHPFGSRTSSVSSQCSMNMNWLSLSLPERPEAPPSYAEVVTEEQRRNNLAPVSACDDFERALQGPLFAYIQEFRFLPPPLYSEIDPNPDQSADDRPSCPSR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96B67 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S207 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| Y382 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot |
研究背景
Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors. Plays a role in NEDD4-mediated ubiquitination and endocytosis af activated ADRB2 and subsequent ADRB2 degradation. May recruit NEDD4 to ADRB2. Alternatively, may function as adapter protein that does not play a major role in recruiting NEDD4 to ADRB2, but rather plays a role in a targeting ADRB2 to endosomes.
Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Lysosome. Endosome. Early endosome.
Note: Associated with plasma membrane, as well as with endosomes and lysosomes during endocytosis (PubMed:16269462, PubMed:23208550, PubMed:20559325).
Highly expressed in skeletal muscle, placenta, kidney, lung, liver, blood, adrenal gland, lymph node, mammary gland, thyroid, and trachea. Very low levels in colon, thymus, spleen, small intestine, bladder and bone marrow. Strong expression in differentiated adipocytes compared to preadipocytes. Detected in omental fat and subcutaneous fat tissue.
Interacts (via PPxY motifs) with NEDD4 (via WW domains). Interacts with ADRB2. Interacts with ADRB3. Interacts with HGS (via PPxY motifs). Does not bind TXN (thioredoxin). Interacts with ITCH. Interacts with WWP1 (via WW domains).
Belongs to the arrestin family.
文献引用
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.