产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0363, RRID:AB_2833528.
展开/折叠
bHLH e33; bHLHe33; Class E basic helix-loop-helix protein 33; RCCP 2; RCCP2; RCCX1; Renal cell carcinoma, papillary; Tcfe 3; Tcfe3; TFE 3; Tfe3; TFE3_HUMAN; TFEA; Transcription factor binding to IGHM enhancer 3; Transcription factor E family, member A; Transcription factor E3; Transcription factor for IgH enhancer; Transcription factor for immunoglobulin heavy chain enhancer 3;
抗原和靶标
- P19532 TFE3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQPLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLPVPPTPGLLSLATTSASDSLKPEQLDIEEEGRPGAATFHVGGGPAQNAPHQQPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILMEEEEGVVGGLSGGALSPLRAASDPLLSSVSPAVSKASSRRSSFSMEEES
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P19532 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K68 | Sumoylation | Uniprot | |
| T85 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| R122 | Methylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| K178 | Methylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| T217 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K330 | Sumoylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| R365 | Methylation | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K401 | Acetylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| S564 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| S568 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot |
研究背景
Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') (By similarity). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (By similarity). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1.
Sumoylated; does not affect dimerization with MITF.
Nucleus.
Ubiquitous in fetal and adult tissues.
Homodimer and heterodimer; with TFEB or MITF.
Belongs to the MiT/TFE family.
研究领域
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
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