产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3546, RRID:AB_2835919.
展开/折叠
CDK5 regulatory subunit associated protein 1 like 1; CDK5 regulatory subunit-associated protein 1-like 1; CDKAL_HUMAN; CDKAL1; Threonylcarbamoyladenosine tRNA methylthiotransferase; tRNA t 6 A37 methylthiotransferase; tRNA-t(6)A37 methylthiotransferase;
抗原和靶标
- Q5VV42 CDKAL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSASCDTLLDDIEDIVSQEDSKPQDRHFVRKDVVPKVRRRNTQKYLQEEENSPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAQEENKKIVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKDNGRRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVDRAKQSFQEGVCEIWLTSEDTGAYGRDIGTNLPTLLWKLVEVIPEGAMLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMEMKREYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKMEQVPAQVKKQRTKDLSRVFHSYSPYDHKIGERQQVLVTEESFDSKFYVAHNQFYEQVLVPKNPAFMGKMVEVDIYESGKHFMKGQPVSDAKVYTPSISKPLAKGEVSGLTKDFRNGLGNQLSSGSHTSAASQCDSASSRMVLPMPRLHQDCALRMSVGLALLGLLFAFFVKVYN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q5VV42 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K37 | Ubiquitination | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| Y315 | Phosphorylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| K488 | Ubiquitination | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| S536 | Phosphorylation | Uniprot |
研究背景
Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
Endoplasmic reticulum membrane>Single-pass membrane protein.
Note: Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
Expressed in pancreatic islets.
Belongs to the methylthiotransferase family. CDKAL1 subfamily.
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