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| 产品: | Cytochrome P450 24A1 抗体 |
| 货号: | DF3568 |
| 描述: | Rabbit polyclonal antibody to Cytochrome P450 24A1 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Rabbit, Dog |
| 分子量: | 60 KD; 59kD(Calculated). |
| 蛋白号: | Q07973 |
| RRID: | AB_2835940 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3568, RRID:AB_2835940.
展开/折叠
1 25 @dihydroxyvitamin D3 24 hydroxylase; 1 25 dihydroxyvitamin D(3) 24 hydroxylase; 1; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24 OHase; 24-OHase; 25-dihydroxyvitamin D(3) 24-hydroxylase; CP 24; CP24; CP24A_HUMAN; CYP 24; CYP24; CYP24A1; Cytochrome P450 24A1; Cytochrome P450 24A1 mitochondrial; cytochrome P450 CC24; Cytochrome P450 family 24; Cytochrome P450 family 24 subfamily A member 1; Cytochrome P450 family 24 subfamily A polypeptide 1; Cytochrome P450 subfamily XXIV; Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase); Cytochrome P450-CC24; EC 1.14.13.n4; Exo mitochondrial protein; HCAI; HCINF1; MGC126273; MGC126274; mitochondrial; P450 CC24; Vitamin D 24 hydroxylase; Vitamin D(3) 24 hydroxylase; Vitamin D(3) 24-hydroxylase;
抗原和靶标
- Q07973 CP24A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSPISKSRSLAAFLQQLRSPRQPPRLVTSTAYTSPQPREVPVCPLTAGGETQNAAALPGPTSWPLLGSLLQILWKGGLKKQHDTLVEYHKKYGKIFRMKLGSFESVHLGSPCLLEALYRTESAYPQRLEIKPWKAYRDYRKEGYGLLILEGEDWQRVRSAFQKKLMKPGEVMKLDNKINEVLADFMGRIDELCDERGHVEDLYSELNKWSFESICLVLYEKRFGLLQKNAGDEAVNFIMAIKTMMSTFGRMMVTPVELHKSLNTKVWQDHTLAWDTIFKSVKACIDNRLEKYSQQPSADFLCDIYHQNRLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQVQQKLLKEIQSVLPENQVPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKATVLGEYALPKGTVLMLNTQVLGSSEDNFEDSSQFRPERWLQEKEKINPFAHLPFGVGKRMCIGRRLAELQLHLALCWIVRKYDIQATDNEPVEMLHSGTLVPSRELPIAFCQR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q07973 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S20 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| K165 | Acetylation | Uniprot | |
| Y220 | Phosphorylation | Uniprot | |
| T388 | Phosphorylation | Uniprot | |
| T395 | Phosphorylation | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| Y407 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot |
研究背景
A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product. Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin.
Mitochondrion.
Belongs to the cytochrome P450 family.
研究领域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Metabolism > Lipid metabolism > Steroid biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
文献引用
Application: WB Species: Human Sample: HK-2 cells
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