产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3578, RRID:AB_2835950.
展开/折叠
4-nitrophenol 2-hydroxylase; CP2E1_HUMAN; CPE1; CYP2E; Cyp2e1; CYPIIE1; Cytochrome P450 2E1; Cytochrome P450 isozyme 3A; Cytochrome P450 LM3A; Cytochrome P450, family 2, subfamily e, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; Cytochrome P450, subfamily IIE; Cytochrome P450-ALC; Cytochrome P450-J; Cytochrome P450RLM6; EC 1.14.13.-; EC 1.14.13.n7; Ethanol-inducible P450; Flavoprotein-linked monooxygenase; MGC133529; Microsomal monooxygenase; OTTHUMP00000020813; OTTHUMP00000046571; P450 ALC; P450-J; P450C2E; P450J; P450RLM6; Xenobiotic monooxygenase;
抗原和靶标
- P05181 CP2E1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSALGVTVALLVWAAFLLLVSMWRQVHSSWNLPPGPFPLPIIGNLFQLELKNIPKSFTRLAQRFGPVFTLYVGSQRMVVMHGYKAVKEALLDYKDEFSGRGDLPAFHAHRDRGIIFNNGPTWKDIRRFSLTTLRNYGMGKQGNESRIQREAHFLLEALRKTQGQPFDPTFLIGCAPCNVIADILFRKHFDYNDEKFLRLMYLFNENFHLLSTPWLQLYNNFPSFLHYLPGSHRKVIKNVAEVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRIPAIKDRQEMPYMDAVVHEIQRFITLVPSNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQEFPDPEKFKPEHFLNENGKFKYSDYFKPFSTGKRVCAGEGLARMELFLLLCAILQHFNLKPLVDPKDIDLSPIHIGFGCIPPRYKLCVIPRS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P05181 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S56 | Phosphorylation | Uniprot | |
| T58 | Phosphorylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| T121 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| T131 | Phosphorylation | Uniprot | |
| T132 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| Y245 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| T373 | Phosphorylation | Uniprot | |
| T376 | Phosphorylation | Uniprot | |
| T387 | Phosphorylation | Uniprot | |
| K410 | Ubiquitination | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| K461 | Ubiquitination | Uniprot |
研究背景
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).
Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein. Mitochondrion inner membrane>Peripheral membrane protein.
Note: Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein.
Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria.
Belongs to the cytochrome P450 family.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Cancers: Overview > Chemical carcinogenesis.
· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.
· Metabolism > Lipid metabolism > Arachidonic acid metabolism.
· Metabolism > Lipid metabolism > Linoleic acid metabolism.
· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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