产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3585, RRID:AB_2835957.
展开/折叠
24 hydroxycholesterol 7 alpha hydroxylase; 24-hydroxycholesterol 7-alpha-hydroxylase; CP39A_HUMAN; Cyp39a1; cytochrome P450 family 39, subfamily A polypeptide 1; Cytochrome P450 39A1; cytochrome P450 subfamily XXXIX (oxysterol 7 alpha hydroxylase) polypeptide 1; Cytochrome P450, family 39, subfamily a, polypeptide 1; hCYP39A1; Oxysterol 7 alpha hydroxylase; Oxysterol 7-alpha-hydroxylase; oxysterol 7alpha hydroxylase;
抗原和靶标
- Q9NYL5 CP39A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELISPTVIIILGCLALFLLLQRKNLRRPPCIKGWIPWIGVGFEFGKAPLEFIEKARIKYGPIFTVFAMGNRMTFVTEEEGINVFLKSKKVDFELAVQNIVYRTASIPKNVFLALHEKLYIMLKGKMGTVNLHQFTGQLTEELHEQLENLGTHGTMDLNNLVRHLLYPVTVNMLFNKSLFSTNKKKIKEFHQYFQVYDEDFEYGSQLPECLLRNWSKSKKWFLELFEKNIPDIKACKSAKDNSMTLLQATLDIVETETSKENSPNYGLLLLWASLSNAVPVAFWTLAYVLSHPDIHKAIMEGISSVFGKAGKDKIKVSEDDLENLLLIKWCVLETIRLKAPGVITRKVVKPVEILNYIIPSGDLLMLSPFWLHRNPKYFPEPELFKPERWKKANLEKHSFLDCFMAFGSGKFQCPARWFALLEVQMCIILILYKYDCSLLDPLPKQSYLHLVGVPQPEGQCRIEYKQRI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NYL5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y102 | Phosphorylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| K386 | Ubiquitination | Uniprot |
研究背景
A cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis. Catalyzes 7-alpha hydroxylation of (24S)-hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Endoplasmic reticulum membrane>Multi-pass membrane protein. Microsome membrane>Multi-pass membrane protein.
Liver specific.
Belongs to the cytochrome P450 family.
研究领域
· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.
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