| 产品: | Cytochrome P450 51A1 抗体 |
| 货号: | DF3591 |
| 描述: | Rabbit polyclonal antibody to Cytochrome P450 51A1 |
| 应用: | WB |
| 反应: | Human |
| 预测: | Pig, Dog |
| 分子量: | 57 KD; 57kD(Calculated). |
| 蛋白号: | Q16850 |
| RRID: | AB_2835963 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3591, RRID:AB_2835963.
展开/折叠
CP51; CP51A_HUMAN; CYP51; CYP51A1; CYPL1; CYPLI; Cytochrome P450 51; Cytochrome P450 51A1; Cytochrome P450 family 51 subfamily A member 1; Cytochrome P450 family 51 subfamily A polypeptide 1; Cytochrome P450, 51 (lanosterol 14 alpha demethylase); Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Lanosterol 14-alpha demethylase; LDM; P450 14DM; P450L1; Sterol 14-alpha demethylase;
抗原和靶标
Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung.
- Q16850 CP51A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLLLGLLQAGGSVLGQAMEKVTGGNLLSMLLIACAFTLSLVYLIRLAAGHLVQLPAGVKSPPYIFSPIPFLGHAIAFGKSPIEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNEDLNAEDVYSRLTTPVFGKGVAYDVPNPVFLEQKKMLKSGLNIAHFKQHVSIIEKETKEYFESWGESGEKNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQKKCYLEQKTVCGENLPPLTYDQLKDLNLLDRCIKETLRLRPPIMIMMRMARTPQTVAGYTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTMIHTPENPVIRYKRRSK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16850 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| K59 | Sumoylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K79 | Sumoylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| Y145 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| R365 | Methylation | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| T370 | Phosphorylation | Uniprot | |
| Y393 | Phosphorylation | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| K436 | Ubiquitination | Uniprot | |
| Y439 | Phosphorylation | Uniprot | |
| Y456 | Phosphorylation | Uniprot |
研究背景
A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25-dihydrolanosterol likely through sequential oxidative conversion of 14-alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Endoplasmic reticulum membrane>Single-pass membrane protein. Microsome membrane>Single-pass membrane protein.
Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung.
Belongs to the cytochrome P450 family.
研究领域
· Metabolism > Lipid metabolism > Steroid biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
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