FGF23 Antibody - #DF3596

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产品: FGF23 抗体
货号: DF3596
描述: Rabbit polyclonal antibody to FGF23
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Dog
分子量: 27 KD; 28kD(Calculated).
蛋白号: Q9GZV9
RRID: AB_2835968

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货期: 当天发货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(91%), Dog(100%)
克隆:
Polyclonal
特异性:
FGF23 Antibody detects endogenous levels of total FGF23.
RRID:
AB_2835968
引用格式: Affinity Biosciences Cat# DF3596, RRID:AB_2835968.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADHR; FGF-23; Fgf23; FGF23_HUMAN; FGFN; Fibroblast growth factor 23; Fibroblast growth factor 23 C-terminal peptide; Fibroblast growth factor 23 precursor; HPDR2; HYPF; Phosphatonin; PHPTC; Tumor derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor;

抗原和靶标

免疫原:
Uniprot:

Q9GZV9(FGF23_HUMAN) >>Visit HPA database.

基因/基因ID:
FGF23(8074)
表达:
Q9GZV9 FGF23_HUMAN:

Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).

序列:
MLGARLRLWVCALCSVCSMSVLRAYPNASPLLGSSWGGLIHLYTATARNSYHLQIHKNGHVDGAPHQTIYSALMIRSEDAGFVVITGVMSRRYLCMDFRGNIFGSHYFDPENCRFQHQTLENGYDVYHSPQYHFLVSLGRAKRAFLPGMNPPPYSQFLSRRNEIPLIHFNTPIPRRHTRSAEDDSERDPLNVLKPRARMTPAPASCSQELPSAEDNSPMASDPLGVVRGGRVNTHAGGTGPEGCRPFAKFI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
100
Horse
91
Rabbit
73
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9GZV9 作为底物

Site PTM Type Enzyme
Y51 Phosphorylation
Y70 Phosphorylation
S71 Phosphorylation
Y154 Phosphorylation
S155 Phosphorylation
S159 Phosphorylation
T178 O-Glycosylation
S180 Phosphorylation Q8IXL6 (FAM20C)
S207 Phosphorylation
S212 Phosphorylation

研究背景

功能:

Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.

翻译修饰:

Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases.

O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23.

细胞定位:

Secreted.
Note: Secretion is dependent on O-glycosylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).

亚基结构:

Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by KL and heparan sulfate glycosaminoglycans that function as coreceptors (By similarity).

蛋白家族:

Belongs to the heparin-binding growth factors family.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles. Bioactive Materials, 2022 (PubMed: 35386445) [IF=18.9]

Application: WB    Species: Rat    Sample:

Fig. 3 Effect of nwHA bioceramics on cocultured osteoporotic osteoblasts. (A) First line: CLSM observations of live (green)/dead (red) staining of osteoporotic osteoblasts cocultured with different nwHA (first line); Second line: CLSM observations of the osteoporotic osteoblasts with F-actin stained with Phalloidin-TRITC (red) and nuclei stained with DAPI (blue). (B) Cell viability of osteoporotic osteoblasts cocultured with different nwHA bioceramics at days 1, 3, and 5. (C) Cell area quantification of osteoporotic osteoblasts from different groups on day 5. (D) qRT-PCR analysis for ATP2A2 and FGF23 gene expressions of osteoporotic osteoblasts from different groups on day 5. (E) Western blotting analysis for ATP2A2 and FGF23 protein expressions of osteoporotic osteoblasts from different groups on day 5; All data are reported as mean ± standard error. ANOVA with Tukey's post hoc test
2). FGF23 alleviates neuronal apoptosis and inflammation, and promotes locomotion recovery via activation of PI3K/AKT signalling in spinal cord injury. Experimental and Therapeutic Medicine, 2023 (PubMed: 37383378) [IF=2.7]

Application: WB    Species: Rat    Sample:

Figure 1 Cell apoptosis, FGF23 and PI3K/AKT signalling in H2O2-stimulated primary neurons. (A) Relative mRNA expression levels, (B) representative western blotting images of FGF23 and (C) FGF23/GAPDH level. (D) Semi-quantification of apoptotic rates and (E) representative flow cytometry plots of Annexin V/PI staining. (F) Representative western blotting images and (G) relative protein expression levels of C-caspase3 and Bcl-2. (H) Representative western blotting images and (I) relative expression ratios of p-PI3K/PI3K and p-AKT/AKT. GAPDH was used as a loading control. *P
3). IL-6, IL-1β and TNF-α regulation of the chondrocyte phenotype: a possible mechanism of haemophilic cartilage destruction. Hematology (Amsterdam, Netherlands), 2023 (PubMed: 36799502) [IF=1.9]

Application: WB    Species: Human    Sample: chondrocytes

Figure 2. IL-6, TNF-α and IL-1β upregulated FGF23 and downregulated SOX9 protein expression in HUM-iCell-s018 chondrocyte cells. Chondrocytes were treated with gradient concentrations of IL-6, TNF-α or IL-1β (0, 1, 5, 10 ng/ml) for 24 h and protein expression of FGF23 and SOX9 in chondrocytes was determined by WB (a – e). Data are expressed with mean ± SD; (b – e) *p 

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