产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3615, RRID:AB_2835987.
展开/折叠
exo1; EXO1_HUMAN; ExoI; Exonuclease 1; Exonuclease I; Exonuclease1; HEX1; hExo I; hExo1; hExoI; Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1;
抗原和靶标
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
- Q9UQ84 EXO1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGIQGLLQFIKEASEPIHVRKYKGQVVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSHGIKPILVFDGCTLPSKKEVERSRRERRQANLLKGKQLLREGKVSEARECFTRSINITHAMAHKVIKAARSQGVDCLVAPYEADAQLAYLNKAGIVQAIITEDSDLLAFGCKKVILKMDQFGNGLEIDQARLGMCRQLGDVFTEEKFRYMCILSGCDYLSSLRGIGLAKACKVLRLANNPDIVKVIKKIGHYLKMNITVPEDYINGFIRANNTFLYQLVFDPIKRKLIPLNAYEDDVDPETLSYAGQYVDDSIALQIALGNKDINTFEQIDDYNPDTAMPAHSRSHSWDDKTCQKSANVSSIWHRNYSPRPESGTVSDAPQLKENPSTVGVERVISTKGLNLPRKSSIVKRPRSAELSEDDLLSQYSLSFTKKTKKNSSEGNKSLSFSEVFVPDLVNGPTNKKSVSTPPRTRNKFATFLQRKNEESGAVVVPGTRSRFFCSSDSTDCVSNKVSIQPLDETAVTDKENNLHESEYGDQEGKRLVDTDVARNSSDDIPNNHIPGDHIPDKATVFTDEESYSFESSKFTRTISPPTLGTLRSCFSWSGGLGDFSRTPSPSPSTALQQFRRKSDSPTSLPENNMSDVSQLKSEESSDDESHPLREEACSSQSQESGEFSLQSSNASKLSQCSSKDSDSEESDCNIKLLDSQSDQTSKLRLSHFSKKDTPLRNKVPGLYKSSSADSLSTTKIKPLGPARASGLSKKPASIQKRKHHNAENKPGLQIKLNELWKNFGFKKDSEKLPPCKKPLSPVRDNIQLTPEAEEDIFNKPECGRVQRAIFQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UQ84 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S68 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| Y284 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| Y375 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| S422 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| T475 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| K482 | Acetylation | Uniprot | |
| K482 | Sumoylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| T502 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| S512 | Phosphorylation | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| K548 | Ubiquitination | Uniprot | |
| T581 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| S639 | Phosphorylation | Uniprot | |
| S659 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| S702 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| T732 | Phosphorylation | Uniprot | |
| K743 | Methylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| T752 | Phosphorylation | Uniprot | |
| K784 | Sumoylation | Uniprot | |
| K796 | Ubiquitination | Uniprot | |
| S804 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| T824 | Phosphorylation | Uniprot |
研究背景
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
Nucleus.
Note: Colocalizes with PCNA to discrete nuclear foci in S-phase.
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
Interacts with the MLH1-PMS2 heterodimer via MLH1. Interacts with MSH3. Interacts with the MSH2-MSH6 heterodimer via MSH2, and this interaction may increase the processivity of the 5'->3' exonuclease activity. Interacts with PCNA, and this interaction may both stimulate the cryptic 3'->5' exonuclease activity and suppress the 5'->3' exonuclease activity. Interacts with WRN, and this interaction stimulates both the 5'->3' exonuclease activity and cleavage of 5'-overhanging flap structures. Interacts with RECQL/RECQ1, and this interaction stimulates cleavage of 5'-overhanging flap structures.
Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
研究领域
· Genetic Information Processing > Replication and repair > Mismatch repair.
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